Loading...
Please wait, while we are loading the content...
Similar Documents
Efficient Isolation of Mycosporine-Like Amino Acids from Marine Red Algae by Fast Centrifugal Partition Chromatography
| Content Provider | MDPI |
|---|---|
| Author | Zwerger, Michael Schwaiger, Stefan Ganzera, Markus |
| Copyright Year | 2022 |
| Description | Marine rhodophyta are known to synthesize specific secondary metabolites, mycosporine-like amino acids (MAAs), to protect themselves from harmful UV-radiation. Shinorine and porphyra-334 are among the most abundant representatives of this compound class. In the present work, a novel approach for their isolation is described. As a first step, a fast centrifugal partition chromatography method, with an aqueous two-phase system comprising water, ethanol, ammonium sulfate and methanol in ascending mode, was developed to isolate the two MAAs from crude aqueous-methanolic extracts of three algal species within 90 min. The compounds could be isolated when just one of them was present in a sample or also both at the same time. By employing solid phase extraction as a second purification step, the individual MAAs were obtained in high purity and good quantity within a much shorter time frame than the established purification protocols, e.g., semi-preparative HPLC. For example, from 4 g Porphyra sp. (Nori) crude extract, 15.7 mg shinorine and 36.2 mg porphyra-334 were isolated. Both were highly pure, as confirmed by TLC, HPLC-MS and NMR analyses. |
| Starting Page | 106 |
| e-ISSN | 16603397 |
| DOI | 10.3390/md20020106 |
| Journal | Marine drugs |
| Issue Number | 2 |
| Volume Number | 20 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-01-27 |
| Access Restriction | Open |
| Subject Keyword | Marine drugs Marine Drugs Analytical Chemistry Mycosporine-like Amino Acids Maa Isolation Fcpc Shinorine Porphyra-334 |
| Content Type | Text |
| Resource Type | Article |
