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Effects of Bacillus Subtilis-Fermented White Sword Bean Extract on Adipogenesis and Lipolysis of 3T3-L1 Adipocytes
| Content Provider | MDPI |
|---|---|
| Author | Choi, Yujeong Kim, Da-Som Lee, Min-Chul Park, Seulgi Lee, Joo-Won Om, Ae-Son |
| Copyright Year | 2021 |
| Description | To investigate the adipogenesis and lipolysis effects of the Bacillus subtilis-fermented white sword bean extract (FWSBE) on 3T3-L1 adipocytes, we treated 3T3-L1 preadipocytes before and after differentiation with FWSBE and measured triglyceride, free glycerol, mRNA, and protein levels. First, FWSBE reduced the cell viability of 3T3-L1 pre-adipocytes under 1000 µg/mL conditions. Triglyceride accumulation in 3T3-L1 pre-adipocytes was suppressed, and free glycerol content in mature 3T3-L1 adipocytes was increased in the FWSBE treatment groups, indicating that FWSBE has anti-obesity effects. Further, FWSBE suppressed adipogenesis in 3T3-L1 pre-adipocytes by lowering the protein levels of C/EBPα, PPARγ, and FAS and increasing the level of pACC and pAMPK. Additionally, FWSBE promoted lipolysis in mature 3T3-L1 adipocytes by increasing the transcription levels of Ppara, Acox, and Lcad and the protein levels of pHSL and ATGL. Thus, we suggest that FWSBE can be a potential dietary supplement because of its anti-obesity properties. |
| Starting Page | 1423 |
| e-ISSN | 23048158 |
| DOI | 10.3390/foods10061423 |
| Journal | Foods |
| Issue Number | 6 |
| Volume Number | 10 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-06-19 |
| Access Restriction | Open |
| Subject Keyword | Foods Endocrinology and Metabolism Canavalia Gladiata Triglyceride Glycerol Amp-activated Protein Kinase Peroxisome Proliferator-activated Receptor |
| Content Type | Text |
| Resource Type | Article |
