NDLI: New Advances in Using Virus-like Particles and Related Technologies for Eukaryotic Genome Editing Delivery

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New Advances in Using Virus-like Particles and Related Technologies for Eukaryotic Genome Editing Delivery

Content Provider	MDPI
Author	Lyu, Pin Lu, Baisong
Copyright Year	2022
Description	The designer nucleases, including Zinc Finger Nuclease (ZFN), Transcription Activator-Like Effector Nuclease (TALEN), and Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas), have been widely used for mechanistic studies, animal model generation, and gene therapy development. Clinical trials using designer nucleases to treat genetic diseases or cancers are showing promising results. Despite rapid progress, potential off-targets and host immune responses are challenges to be addressed for in vivo uses, especially in clinical applications. Short-term expression of the designer nucleases is necessary to reduce both risks. Currently, delivery methods enabling transient expression of designer nucleases are being pursued. Among these, virus-like particles as delivery vehicles for short-term designer nuclease expression have received much attention. This review will summarize recent developments in using virus-like particles (VLPs) for safe delivery of gene editing effectors to complement our last review on the same topic. First, we introduce some background information on how VLPs can be used for safe and efficient CRISPR/Cas9 delivery. Then, we summarize recently developed virus-like particles as genome editing vehicles. Finally, we discuss applications and future directions.
Starting Page	8750
e-ISSN	14220067
DOI	10.3390/ijms23158750
Journal	International Journal of Molecular Sciences
Issue Number	15
Volume Number	23
Language	English
Publisher	MDPI
Publisher Date	2022-08-06
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Immunology Virus-like Particle (vlp) Viral Capsid Aptamer Aptamer-binding Protein Genome Editing Designer Nuclease Delivery Rna Ribonucleoprotein Zfn Talen Crispr/cas9
Content Type	Text
Resource Type	Article

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