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The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure
| Content Provider | MDPI |
|---|---|
| Author | Jan, Jastrzębski Koniuszewska, Izabela Harnisz, Monika Korzeniewska, Ewa Czatzkowska, Małgorzata Paukszto, Łukasz Bajkacz, Sylwia Felis, Ewa Rusanowska, Paulina |
| Copyright Year | 2021 |
| Description | This study explored the effect of eight antimicrobials on the efficiency of biogas production in the anaerobic digestion (AD) process of cattle manure. The microbiome involved in AD, presence and number of genes mcrA, MSC and MST specific for Archaea, and antibiotic resistance genes (ARGs) concentration in digestate (D) were examined. Supplementation of antibiotics to substrate significantly lowered biogas production. Amoxicillin caused a 75% decrease in CH4 production in comparison with the control samples. Enrofloxacin, tetracycline, oxytetracycline, and chlortetracycline reduced the amount of biogas produced by 36, 39, 45 and 53%, respectively. High-throughput sequencing of 16S rRNA results revealed that bacteria dominated the Archaea microorganisms in all samples. Moreover, antibiotics led to a decrease in the abundance of the genes mcrA, MSC, MST, and induced an increase in the number of tetracyclines resistance genes. Antibiotics decreased the efficiency of the AD process and lowered the quantity of CH4 obtained, while stimulating an increase in the number of ARGs in D. This work reveals how antimicrobials affect the cattle manure AD process and changes in microbial biodiversity, number of functional genes and ARGs in the digestate due to drugs exposure. It also, provides useful, practical information about the AD process. |
| Starting Page | 1125 |
| e-ISSN | 19961073 |
| DOI | 10.3390/en14041125 |
| Journal | Energies |
| Issue Number | 4 |
| Volume Number | 14 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-02-20 |
| Access Restriction | Open |
| Subject Keyword | Energies Water Science and Technology Mesophilic Anaerobic Digestion Biogas Production Microbiome Biodiversity Antibiotic Resistance Genes Illumina Miseq Sequencing |
| Content Type | Text |
| Resource Type | Article |
