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Isolation and Aflatoxin B1-Degradation Characteristics of a Microbacterium proteolyticum B204 Strain from Bovine Faeces
| Content Provider | MDPI |
|---|---|
| Author | Yan, Yi Zhang, Xinyue Chen, Haiyan Huang, Wenmin Jiang, Hongnian Wang, Chulun Xiao, Zhuang Zhang, Yuyu Xu, Jialiang |
| Copyright Year | 2022 |
| Description | Aflatoxin $B_{1}$ $(AFB_{1}$) is one of the most harmful mycotoxins, raising serious global health and economic problems. Searching for biological approaches for effective and safe $AFB_{1}$ degradation is imminent. In our study, Microbacterium proteolyticum B204 isolated from bovine faeces degraded 77% of $AFB_{1}$ after 24 h, becoming the first reported bacteria from the Microbacterium family to possess $AFB_{1}$ degradation characteristics. Temperature variation showed little effect on its degradation ratio, demonstrating high thermostability of 75% and 79% after boiling and sterilization, respectively. We suppose that the components playing a key role during this process were proteins, considering the decreased degradation rate caused by Proteinase K. Cell viability detection on HepG2 cells indicated that the degradation products were much less toxic than pure $AFB_{1}$. Furthermore, B204 cell-free culture supernatant also degraded $AFB_{1}$-contaminated food, such as peanuts, corn and cheese. These results suggested that this strain with $AFB_{1}$ degradation properties could be a prospective candidate for application in the food and feed industries. |
| Starting Page | 525 |
| e-ISSN | 20726651 |
| DOI | 10.3390/toxins14080525 |
| Journal | Toxins |
| Issue Number | 8 |
| Volume Number | 14 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-07-30 |
| Access Restriction | Open |
| Subject Keyword | Toxins Afb1 Microbacterium Proteolyticum Degradation Detoxification |
| Content Type | Text |
| Resource Type | Article |
