NDLI: Advanced Maternal Age Deteriorates the Developmental Competence of Vitrified Oocytes in Mice

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Advanced Maternal Age Deteriorates the Developmental Competence of Vitrified Oocytes in Mice

Content Provider	MDPI
Author	Lee, Ju Park, Jae Yoon, Sook Park, Eun Jun, Jin Lim, Hyunjung Kim, Jayeon Song, Haengseok
Copyright Year	2021
Description	Advanced maternal age (AMA) is known to be related to the decrease in the quality and quantity of oocytes. Oocyte vitrification is now considered an established assisted reproductive technology for fertility preservation. However, it remains unclear whether the oocytes in older women are more sensitive to various insults during vitrification. Thus, we evaluated whether AMA affects cellular and molecular features and developmental outcomes of oocytes after vitrification in mice. The oocytes were grouped as young fresh (YF), young vitrified/warmed (YV), aged fresh (AF), and aged vitrified/warmed (AV). The survival rate of AV oocytes was significantly lower than that of YV oocytes. The rates of fertilization, cleavage, and blastocyst formation of AV oocytes were significantly lower than those of other groups. AV oocytes were represented as aberrations in mitochondria distribution, microvacuole size, and autophagosome formation, leading to delayed embryo development in mice. This delay was associated with a reduced number of total cells and trophectoderm in the blastocyst developed from AV oocytes. Collectively, AMA exaggerates the vulnerability of oocytes to cryo-damage that occurs during vitrification in mice, suggesting that the current vitrification protocols optimized for oocytes from young females should be modified for oocytes from aged women.
Starting Page	1563
e-ISSN	20734409
DOI	10.3390/cells10061563
Journal	Cells
Issue Number	6
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2021-06-21
Access Restriction	Open
Subject Keyword	Cells Reproductive Biology Advanced Maternal Age (ama) Oocyte Vitrification Oocyte Quality Developmental Competence Fertility Preservation Time-lapse Monitoring
Content Type	Text
Resource Type	Article

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