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Functional Voltage-Gated Sodium Channels Are Present in the Human B Cell Membrane
| Content Provider | MDPI |
|---|---|
| Author | Marianna, Pócsi Feher, Adam Papp, Ferenc Szanto, Tibor G. Csoti, Agota Fejes, Zsolt Nagy, Béla Nemes, Balázs Varga, Zoltan |
| Copyright Year | 2022 |
| Description | B cells express various ion channels, but the presence of voltage-gated sodium $(Na_{V}$) channels has not been confirmed in the plasma membrane yet. In this study, we have identified several $Na_{V}$ channels, which are expressed in the human B cell membrane, by electrophysiological and molecular biology methods. The sensitivity of the detected sodium current to tetrodotoxin was between the values published for TTX-sensitive and TTX-insensitive channels, which suggests the co-existence of multiple $Na_{V}$1 subtypes in the B cell membrane. This was confirmed by RT-qPCR results, which showed high expression of TTX-sensitive channels along with the lower expression of TTX-insensitive $Na_{V}$1 channels. The biophysical characteristics of the currents also supported the expression of multiple $Na_{V}$ channels. In addition, we investigated the potential functional role of $Na_{V}$ channels by membrane potential measurements. Removal of $Na^{+}$ from the extracellular solution caused a reversible hyperpolarization, supporting the role of $Na_{V}$ channels in shaping and maintaining the resting membrane potential. As this study was mainly limited to electrophysiological properties, we cannot exclude the possible non-canonical functions of these channels. This work concludes that the presence of voltage-gated sodium channels in the plasma membrane of human B cells should be recognized and accounted for in the future. |
| Starting Page | 1225 |
| e-ISSN | 20734409 |
| DOI | 10.3390/cells11071225 |
| Journal | Cells |
| Issue Number | 7 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-04-05 |
| Access Restriction | Open |
| Subject Keyword | Cells Immunology B Cell Lymphocytes Voltage-gated Sodium Channel Patch-clamp Electrophysiology Tetrodotoxin |
| Content Type | Text |
| Resource Type | Article |
