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A Portable Nanoprobe for Rapid and Sensitive Detection of SARS-CoV-2 S1 Protein
| Content Provider | MDPI |
|---|---|
| Author | Alhadrami, Hani A. Suaifan, Ghadeer A. R. Y. Zourob, Mohammed M. |
| Copyright Year | 2022 |
| Description | Simple, timely, and precise detection of SARS-CoV-2 in clinical samples and contaminated surfaces aids in lowering attendant morbidity/mortality related to this infectious virus. Currently applied diagnostic techniques depend on a timely laboratory report following PCR testing. However, the application of these tests is associated with inherent shortcomings due to the need for trained personnel, long-time centralized laboratories, and expensive instruments. Therefore, there is an interest in developing biosensing diagnostic frontiers that can help in eliminating these shortcomings with a relatively economical, easy-to-use, well-timed, precise and sensitive technology. This study reports the development of fabricated Q-tips designed to qualitatively and semi-quantitatively detect SARS-CoV-2 in clinical samples and contaminated non-absorbable surfaces. This colorimetric sensor is engineered to sandwich SARS-CoV-2 spike protein between the lactoferrin general capturing agent and the complementary ACE2-labeled receptor. The ACE2 receptor is decorated with an orange-colored polymeric nanoparticle to generate an optical visual signal upon pairing with the SARS-CoV-2 spike protein. This colorimetric change of the Q-tip testing zone from white to orange confirms a positive result. The visual detection limit of the COVID-19 engineered colorimetric Q-tip sensor was 100 pfu/mL within a relatively short turnaround time of 5 min. The linear working range of quantitation was $10^{3}–10^{8}$ pfu/mL. The engineered sensor selectively targeted SARS-CoV-2 spike protein and did not bind to another coronavirus such as MERS-CoV, Flu A, or Flu B present on the contaminated surface. This novel detection tool is relatively cheap to produce and suitable for onsite detection of COVID-19 infection. |
| Starting Page | 232 |
| e-ISSN | 20796374 |
| DOI | 10.3390/bios12040232 |
| Journal | Biosensors |
| Issue Number | 4 |
| Volume Number | 12 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-04-11 |
| Access Restriction | Open |
| Subject Keyword | Biosensors Analytical Chemistry Sars-cov-2 Covid-19 Rapid Biosensors Onsite Detection Low-cost Diagnostic Assay |
| Content Type | Text |
| Resource Type | Article |
