NDLI: Phenolic Acids from Lycium barbarum leaves: In Vitro and In Silico Studies of the Inhibitory Activity Against Porcine Pancreatic α-Amylase

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Phenolic Acids from Lycium barbarum leaves: In Vitro and In Silico Studies of the Inhibitory Activity Against Porcine Pancreatic α-Amylase

Content Provider	MDPI
Author	Jordi, Mañes Pollini, Luna Riccio, Alessandra Juan, Cristina Tringaniello, Carmela Ianni, Federica Blasi, Francesca Macchiarulo, Antonio Cossignani, Lina
Copyright Year	2020
Description	Nowadays, bioactive compounds from vegetable food and waste are of great interest for their inhibitory potential against digestive enzymes. In the present study, the inhibitory activity of methanolic extract from Lycium barbarum leaves on porcine pancreas α-amylase has been studied. The α-amylase inhibitory activity of the constituent phenolic acids was also investigated. The leaves were extracted by ultrasound-assisted method, one of the most efficient techniques for bioactive extraction from plant materials, and then the phenolic acids were identified by Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) Liquid Chromatography/Mass Spectrometry (LC/MS). Chlorogenic and salicylic acids were the most abundant phenolic acids in L. barbarum leaf extract. The inhibitory effect against α-amylase, determined for individual compounds by in vitro assay, was higher for chlorogenic, salicylic, and caffeic acids. L. barbarum leaf extract showed an appreciable α-amylase inhibitory effect in a concentration-dependent manner. Docking studies of the considered phenolic acids into the active site of α-amylase suggested a conserved binding mode that is mainly stabilized through H-bonds and π-π stacking interactions.
Starting Page	1388
e-ISSN	22279717
DOI	10.3390/pr8111388
Journal	Processes
Issue Number	11
Volume Number	8
Language	English
Publisher	MDPI
Publisher Date	2020-10-30
Access Restriction	Open
Subject Keyword	Processes Integrative and Complementary Medicine Hypoglycaemic Activity Molecular Docking Goji Leaves Bioactives Antioxidant Activity Food Waste Uae
Content Type	Text
Resource Type	Article

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