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Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth
| Content Provider | MDPI |
|---|---|
| Author | Laomeephol, Chavee Vasuratna, Apichai Ratanavaraporn, Juthamas Kanokpanont, Sorada Luckanagul, Jittima Amie Humenik, Martin Scheibel, Thomas Damrongsakkul, Siriporn |
| Copyright Year | 2021 |
| Description | Binary-blended hydrogels fabricated from Bombyx mori silk fibroin (SF) and recombinant spider silk protein eADF4(C16) were developed and investigated concerning gelation and cellular interactions in vitro. With an increasing concentration of eADF4(C16), the gelation time of SF was shortened from typically one week to less than 48 h depending on the blending ratio. The biological tests with primary cells and two cell lines revealed that the cells cannot adhere and preferably formed cell aggregates on eADF4(C16) hydrogels, due to the polyanionic properties of eADF4(C16). Mixing SF in the blends ameliorated the cellular activities, as the proliferation of L929 fibroblasts and SaOS-2 osteoblast-like cells increased with an increase of SF content. The blended SF:eADF4(C16) hydrogels attained the advantages as well as overcame the limitations of each individual material, underlining the utilization of the hydrogels in several biomedical applications. |
| Starting Page | 4182 |
| e-ISSN | 20734360 |
| DOI | 10.3390/polym13234182 |
| Journal | Polymers |
| Issue Number | 23 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-11-29 |
| Access Restriction | Open |
| Subject Keyword | Polymers Silk Fibroin Spider Silk Hydrogel Self-assembly Cell Culture |
| Content Type | Text |
| Resource Type | Article |
