NDLI: Molecular Evidence for Flea-Borne Rickettsiosis in Febrile Patients from Madagascar

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Molecular Evidence for Flea-Borne Rickettsiosis in Febrile Patients from Madagascar

Content Provider	MDPI
Author	Keller, Christian Rakotozandrindrainy, Raphaël von Kalckreuth, Vera Heriniaina, Jean Noël Schwarz, Norbert Georg Pak, Gi Deok Im, Justin Espinoza, Ligia Maria Cruz Hagen, Ralf Matthias Frickmann, Hagen Rakotondrainiarivelo, Jean Philibert Razafindrabe, Tsiry Dekker, Denise May, Jürgen Poppert, Sven Marks, Florian
Copyright Year	2021
Description	Rickettsiae may cause febrile infections in humans in tropical and subtropical regions. From Madagascar, no molecular data on the role of rickettsioses in febrile patients are available. Blood samples from patients presenting with fever in the area of the capital Antananarivo were screened for the presence of rickettsial DNA. EDTA (ethylenediaminetetraacetic acid) blood from 1020 patients presenting with pyrexia > 38.5 °C was analyzed by gltA-specific qPCR. Positive samples were confirmed by ompB-specific qPCR. From confirmed samples, the gltA amplicons were sequenced and subjected to phylogenetic analysis. From five gltA-reactive samples, two were confirmed by ompB-specific qPCR. The gltA sequence in the sample taken from a 38-year-old female showed 100% homology with R. typhi. The other sample taken from a 1.5-year-old infant was 100% homologous to R. felis. Tick-borne rickettsiae were not identified. The overall rate of febrile patients with molecular evidence for a rickettsial infection from the Madagascan study site was 0.2% (2/1020 patients). Flea-borne rickettsiosis is a rare but neglected cause of infection in Madagascar. Accurate diagnosis may prompt adequate antimicrobial treatment.
Starting Page	1482
e-ISSN	20760817
DOI	10.3390/pathogens10111482
Journal	Pathogens
Issue Number	11
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2021-11-14
Access Restriction	Open
Subject Keyword	Pathogens Infectious Diseases Rickettsia Typhi Rickettsia Felis Madagascar Flea-borne Rickettsiosis Murine Typhus
Content Type	Text
Resource Type	Article

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