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Ultrasound-Assisted Water Extraction of Mastocarpus stellatus Carrageenan with Adequate Mechanical and Antiproliferative Properties
| Content Provider | MDPI |
|---|---|
| Author | Noelia, Flórez-Fernández Torres, Maria Dominguez, Herminia |
| Copyright Year | 2021 |
| Description | Ultrasound-assisted water extraction was optimized to recover gelling biopolymers and antioxidant compounds from Mastocarpus stellatus. A set of experiments following a Box–Behnken design was proposed to study the influence of extraction time, solid liquid ratio, and ultrasound amplitude on the yield, sulfate content, and thermo-rheological properties (viscoelasticity and gelling temperature) of the carrageenan fraction, as well as the composition (protein and phenolic content) and antiradical capacity of the soluble extracts. Operating at 80 °C and 80 kHz, the models predicted a compromise optimum extraction conditions at ~35 min, solid liquid ratio of ~2 g/100 g, and ultrasound amplitude of ~79%. Under these conditions, 40.3% carrageenan yield was attained and this product presented 46% sulfate and good mechanical properties, a viscoelastic modulus of 741.4 Pa, with the lowest gelling temperatures of 39.4 °C. The carrageenans also exhibited promising antiproliferative properties on selected human cancer cellular lines, A-549, A-2780, HeLa 229, and HT-29 with $EC_{50}$ under 51.9 μg/mL. The dried soluble extract contained 20.4 mg protein/g, 11.3 mg gallic acid eq/g, and the antiradical potency was equivalent to 59 mg Trolox/g. |
| Starting Page | 280 |
| e-ISSN | 16603397 |
| DOI | 10.3390/md19050280 |
| Journal | Marine drugs |
| Issue Number | 5 |
| Volume Number | 19 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-05-19 |
| Access Restriction | Open |
| Subject Keyword | Marine drugs Marine Drugs Integrative and Complementary Medicine Paper and Wood Box–behnken Design Hybrid Carrageenan Antioxidants Antiproliferative Biopolymer |
| Content Type | Text |
| Resource Type | Article |
