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Identification and Fine-Mapping of Clubroot (Plasmodiophora brassicae) Resistant QTL in Brassica rapa
| Content Provider | MDPI |
|---|---|
| Author | Zhang, Hui Ma, Xiaochao Liu, Xitong Zhang, Shifan Li, Fei Li, Guoliang Sun, Rifei Zhang, Shujiang |
| Copyright Year | 2022 |
| Description | European fodder turnips (Brassica rapa ssp. rapifera) were identified as sources of clubroot resistance (CR) and have been widely used in Brassica resistance breeding. An $F_{2}$ population derived from a cross between a resistant turnip and a susceptible Chinese cabbage was used to determine the inheritance and locating the resistance Quantitative Trait Loci (QTLs). The parents showed to be very resistant/susceptible to the field isolates (pathotype 4) of clubroot from Henan in China. After inoculation, 27 very resistant or susceptible individuals were selected to construct bulks, respectively. Next-generation-sequencing-based Bulk Segregant Analysis Sequencing (BSA-Seq) was used and located resistance QTL on chromosome A03 (3.3–7.5 Mb) and A08 (0.01–6.5 Mb), named Bcr1 and Bcr2, respectively. Furthermore, an $F_{3}$ population including 180 families derived from $F_{2}$ individuals was phenotyped and used to verify and narrow candidate regions. Ten and seven Kompetitive Allele-Specific PCR (KASP) markers narrowed the target regions to 4.3–4.78 Mb (A03) and 0.02–0.79 Mb (A08), respectively. The phenotypic variation explained (PVE) of the two QTLs were 33.3% and 13.3% respectively. The two candidate regions contained 99 and 109 genes. In the A03 candidate region, there were three candidate R genes, namely Bra006630, Bra006631 and Bra006632. In the A08 candidate region, there were two candidate R genes, namely Bra030815 and Bra030846. |
| Starting Page | 66 |
| e-ISSN | 23117524 |
| DOI | 10.3390/horticulturae8010066 |
| Journal | Horticulturae |
| Issue Number | 1 |
| Volume Number | 8 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-01-11 |
| Access Restriction | Open |
| Subject Keyword | Horticulturae Horticulture Plasmodiophora Brassicae Brassica Rapa Qtl Resistant Genes Bsa-seq Fine-mapping |
| Content Type | Text |
| Resource Type | Article |
