NDLI: Stepwise Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells in Collagen Sponges under Different Microenvironments

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Stepwise Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells in Collagen Sponges under Different Microenvironments

Content Provider	MDPI
Author	Zheng, Jing Xie, Yan Yoshitomi, Toru Kawazoe, Naoki Yang, Yingnan Chen, Guoping
Copyright Year	2022
Description	Biomimetic microenvironments are important for controlling stem cell functions. In this study, different microenvironmental conditions were investigated for the stepwise control of proliferation and chondrogenic differentiation of human bone-marrow-derived mesenchymal stem cells (hMSCs). The hMSCs were first cultured in collagen porous sponges and then embedded with or without collagen hydrogels for continual culture under different culture conditions. The different influences of collagen sponges, collagen hydrogels, and induction factors were investigated. The collagen sponges were beneficial for cell proliferation. The collagen sponges also promoted chondrogenic differentiation during culture in chondrogenic medium, which was superior to the effect of collagen sponges embedded with hydrogels without loading of induction factors. However, collagen sponges embedded with collagen hydrogels and loaded with induction factors had the same level of promotive effect on chondrogenic differentiation as collagen sponges during in vitro culture in chondrogenic medium and showed the highest promotive effect during in vivo subcutaneous implantation. The combination of collagen sponges with collagen hydrogels and induction factors could provide a platform for cell proliferation at an early stage and subsequent chondrogenic differentiation at a late stage. The results provide useful information for the chondrogenic differentiation of stem cells and cartilage tissue engineering.
Starting Page	6406
e-ISSN	14220067
DOI	10.3390/ijms23126406
Journal	International Journal of Molecular Sciences
Issue Number	12
Volume Number	23
Language	English
Publisher	MDPI
Publisher Date	2022-06-08
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Cell Tissue Engineering Collagen Sponge Hydrogel Chondrogenesis Induction Factor Mesenchymal Stem Cells Proliferation Chondrogenesis Cartilage Tissue Engineering
Content Type	Text
Resource Type	Article

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