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Tyramine-Functionalized Alginate-Collagen Hybrid Hydrogel Inks for 3D-Bioprinting
| Content Provider | MDPI |
|---|---|
| Author | Kim, Sung Dong Jin, Subin Kim, Sumin Son, Donghee Shin, Mikyung |
| Copyright Year | 2022 |
| Description | Extrusion-based 3D-bioprinting using hydrogels has exhibited potential in precision medicine; however, researchers are beset with several challenges. A major challenge of this technique is the production of constructs with sufficient height and fidelity to support cellular behavior in vivo. In this study, we present the 3D-bioprinting of cylindrical constructs with tunable gelation kinetics by controlling the covalent crosslinking density and gelation time of a tyramine-functionalized alginate hydrogel (ALG-TYR) via enzymatic reaction by horseradish peroxidase (HRP) and hydrogen peroxide $(H_{2}O_{2}$). The extruded filament was crosslinked for a second time on a support bath containing $H_{2}O_{2}$ to increase fidelity after printing. The resulting tubular construct, with a height of 6 mm and a wall thickness of 2 mm, retained its mechanical properties and had a maximum 2-fold swelling after 2 d. Furthermore, collagen (COL) was introduced into the ALG-TYR hydrogel network to increase the mechanical modulus and cell cytocompatibility, as the encapsulated fibroblast cells exhibited a higher cell viability in the ALG-TYR/COL construct (92.13 ± 0.70%) than in ALG-TYR alone (68.18 ± 3.73%). In summary, a vascular ECM-mimicking scaffold was 3D-bioprinted with the ALG-TYR/COL hybrid hydrogel, and this scaffold can support tissue growth for clinical translation in regenerative and personalized medicine. |
| Starting Page | 3173 |
| e-ISSN | 20734360 |
| DOI | 10.3390/polym14153173 |
| Journal | Polymers |
| Issue Number | 15 |
| Volume Number | 14 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-08-03 |
| Access Restriction | Open |
| Subject Keyword | Polymers Cell Tissue Engineering 3d-bioprinting Tyramine-conjugated Alginate Collagen Hybrid Hydrogel Bioink |
| Content Type | Text |
| Resource Type | Article |
