NDLI: Next-Generation Sequencing in Lung Cancer Patients: A Comparative Approach in NSCLC and SCLC Mutational Landscapes

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Next-Generation Sequencing in Lung Cancer Patients: A Comparative Approach in NSCLC and SCLC Mutational Landscapes

Content Provider	MDPI
Author	Antonia, Haranguș Pop-Bica, Cecilia Ciocan, Cristina Alexandra Braicu, Cornelia Simon, Marioara Nutu, Andreea Pop, Laura Ancuta Slaby, Ondrej Atanasov, Atanas G. Pirlog, Radu Hajjar, Nadim Al Berindan-Neagoe, Ioana
Copyright Year	2022
Description	Background: Lung cancer remains one of the most diagnosed malignancies, being the second most diagnosed cancer, while still being the leading cause of cancer-related deaths. Late diagnosis remains a problem, alongside the high mutational burden encountered in lung cancer. Methods: We assessed the genetic profile of cancer genes in lung cancer using The Cancer Genome Atlas (TCGA) datasets for mutations and validated the results in a separate cohort of 32 lung cancer patients using tumor tissue and whole blood samples for next-generation sequencing (NGS) experiments. Another separate cohort of 32 patients was analyzed to validate some of the molecular alterations depicted in the NGS experiment. Results: In the TCGA analysis, we identified the most commonly mutated genes in each lung cancer dataset, with differences among the three histotypes analyzed. NGS analysis revealed TP53, CSF1R, PIK3CA, FLT3, ERBB4, and KDR as being the genes most frequently mutated. We validated the c.1621A>C mutation in KIT. The correlation analysis indicated negative correlation between adenocarcinoma and altered PIK3CA (r = −0.50918; p = 0.0029). TCGA survival analysis indicated that NRAS and IDH2 (LUAD), STK11 and TP53 (LUSC), and T53 (SCLC) alterations are correlated with the survival of patients. Conclusions: The study revealed differences in the mutational landscape of lung cancer histotypes.
Starting Page	453
e-ISSN	20754426
DOI	10.3390/jpm12030453
Journal	Journal of Personalized Medicine
Issue Number	3
Volume Number	12
Language	English
Publisher	MDPI
Publisher Date	2022-03-13
Access Restriction	Open
Subject Keyword	Journal of Personalized Medicine Oncology Non-small-cell Lung Cancer Small-cell Lung Cancer Targeted Sequencing Patients
Content Type	Text
Resource Type	Article

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