NDLI: Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages

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Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages

Content Provider	MDPI
Author	Jeong, Dabin Ko, Wan-Kyu Kim, Seong-Jun Han, Gong-Ho Lee, Daye Sheen, Seung-Hun Sohn, Seil
Copyright Year	2021
Description	The purpose of this study is to elucidate the anti-inflammatory effect of lobeglitazone (LOBE) in lipopolysaccharide (LPS)-induced bone-marrow derived macrophages (BMDMs). We induced nitric oxide (NO) production and pro-inflammatory gene expression through LPS treatment in BMDMs. The changes of NO release and expression of pro-inflammatory mediators by LOBE were assessed via NO quantification assay and a real-time quantitative polymerase chain reaction (RT-qPCR), respectively. In addition, the regulatory effect of LOBE on activation of mitogen-activated protein kinase (MAPK) signaling pathway was investigated by measuring the phosphorylation state of extracellular regulatory protein (ERK) and c-Jun N-terminal kinase (JNK) proteins by Western blot. Our results show that LOBE significantly reduced LPS-induced NO production and pro-inflammatory gene expression of interleukin-1β (IL-1β), IL-6, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and monocyte chemoattractant protein-1 (MCP-1). Moreover, LOBE reduced phosphorylation levels of ERK and JNK of MAPK signaling pathway. In conclusion, LOBE exerts an anti-inflammatory effect in LPS-induced BMDMs by suppression of NO production and pro-inflammatory gene expression, and this effect is potentially through inhibition of the MARK signaling pathway.
Starting Page	1432
e-ISSN	22279059
DOI	10.3390/biomedicines9101432
Journal	Biomedicines
Issue Number	10
Volume Number	9
Language	English
Publisher	MDPI
Publisher Date	2021-10-10
Access Restriction	Open
Subject Keyword	Biomedicines Dentistry and Oral Surgery Bone-marrow Derived Macrophages Lipopolysaccharide Lobeglitazone Anti-inflammation Mitogen-activated Protein Kinase
Content Type	Text
Resource Type	Article

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