NDLI: Improving the Efficiency of Adventitious Shoot Induction and Somatic Embryogenesis via Modification of WUSCHEL and LEAFY COTYLEDON 1

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Improving the Efficiency of Adventitious Shoot Induction and Somatic Embryogenesis via Modification of WUSCHEL and LEAFY COTYLEDON 1

Content Provider	MDPI
Author	Ikeda, Miho Takahashi, Mikiya Fujiwara, Sumire Mitsuda, Nobutaka Ohme-Takagi, Masaru
Copyright Year	2020
Description	The induction of adventitious organs, such as calli, shoots, and somatic embryos, in tissue culture is a useful technique for plant propagation and genetic modification. In recent years, several genes have been reported to be adventitious organ inducers and proposed to be useful for industrial applications. Even though the Arabidopsis (Arabidopsis thaliana) WUSCHEL (WUS) and LEAFY COTYLEDON 1 (LEC1) genes can induce adventitious organ formation in Arabidopsis without phytohormone treatment, further improvement is desired. Here, we show that modifying the transcriptional repression/activation activities of WUS and LEC1 improves the efficiency of adventitious organ formation in Arabidopsis. Because WUS functions as a transcriptional repressor during the induction of adventitious organs, we fused it to an artificial strong repression domain, SUPERMAN REPRESSION DOMAIN X (SRDX). Conversely, we fused the strong transcriptional activation domain VP16 from herpes simplex virus to LEC1. Upon overexpression of the corresponding transgenes, we s쳮ded in improving the efficiency of adventitious organ induction. Our results show that the modification of transcriptional repression/activation activity offers an effective method to improve the efficiency of adventitious organ formation in plants.
Starting Page	1434
e-ISSN	22237747
DOI	10.3390/plants9111434
Journal	Plants
Issue Number	11
Volume Number	9
Language	English
Publisher	MDPI
Publisher Date	2020-10-25
Access Restriction	Open
Subject Keyword	Plants Plant Sciences Somatic Embryogenesis Adventitious Shoot Formation Transcription Factor Stem Cell
Content Type	Text
Resource Type	Article

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