| Content Provider | MDPI |
|---|---|
| Author | Lai, Xing Gao, Gan Watanabe, Junji Liu, Huiyu Shen, Heyun |
| Abstract | In this study, polyelectrolyte multilayers were fabricated on a polystyrene (PS) plate using a Layer-by-Layer (LbL) self-assembly technique. The resulting functional platform showed improved performance compared with conventional enzyme-linked immunosorbent assay (ELISA) systems. Poly(diallyldimethylammonium chloride) (PDDA) and poly(acrylic acid) (PAA) were used as cationic and anionic polyelectrolytes. On the negatively-charged (PDDA/PAA)3 polyelectrolyte multilayers the hydrophilic PAA surface could efficiently decrease the magnitude of the noise signal, by inhibiting nonspecific adsorption even without blocking reagent adsorption. Moreover, the (PDDA/PAA)3 substrate covalently immobilized the primary antibody, greatly increasing the amount of primary antibody adsorption and enhancing the specific detection signal compared with a conventional PS plate. The calibration curve of the (PDDA/PAA)3 substrate showed a wide linear range, for concentrations from 0.033 to 33 nM, a large specific signal change, and a detection limit of 33 pM, even though the conventional blocking reagent adsorption step was omitted. The (PDDA/PAA)3 substrate provided a high-performance ELISA system with a simple fabrication process and high sensitivity; the system presented here shows potential for a variety of immunosensor applications. |
| File Size | 2001920 |
| File Format | |
| e-ISSN | 20734360 |
| DOI | 10.3390/polym9020051 |
| Journal | Polymers |
| Issue Number | 2 |
| Volume Number | 9 |
| Language | English |
| Publisher Date | 2017-02-12 |
| Access Restriction | Open |
| Subject Keyword | polyelectrolyte multilayers ELISA poly(acrylic acid) hydrophilic surface |
| Content Type | Text |
| Resource Type | Article |
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