NDLI: A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction

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Volume: 16

Volume: 15

Integrated (Meta) Genomic and Synthetic Biology Approaches to Develop New Biocatalysts

Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi

Looking Beyond the Terrestrial: The Potential of Seaweed Derived Bioactives to Treat Non-Communicable Diseases

Marine Natural Products from New Caledonia—A Review

Aspergillus Sydowii Marine Fungal Bloom in Australian Coastal Waters, Its Metabolites and Potential Impact on Symbiodinium Dinoflagellates

Discovery of Novel Antiangiogenic Marine Natural Product Scaffolds

Astaxanthin Inhibits Acetaldehyde-Induced Cytotoxicity in SH-SY5Y Cells by Modulating Akt/CREB and p38MAPK/ERK Signaling Pathways

Pseudopterosin A: Protection of Synaptic Function and Potential as a Neuromodulatory Agent

Deep Subseafloor Fungi as an Untapped Reservoir of Amphipathic Antimicrobial Compounds

Nigrodiquinone A, a Hydroanthraquinone Dimer Containing a Rare C-9–C-7′ Linkage from a Zoanthid-Derived Nigrospora sp. Fungus

Comparison of Protein Phosphatase Inhibition Assay with LC-MS/MS for Diagnosis of Microcystin Toxicosis in Veterinary Cases

Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum

Antimicrobial Action of Compounds from Marine Seaweed

Bioprospecting of Marine Macrophytes Using MS-Based Lipidomics as a New Approach

Antimicrobials from Cnidarians. A New Perspective for Anti-Infective Therapy?

Eutypenoids A–C: Novel Pimarane Diterpenoids from the Arctic Fungus Eutypella sp. D-1

Chinese Marine Materia Medica Resources: Status and Potential

A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction

Sulfated Seaweed Polysaccharides as Multifunctional Materials in Drug Delivery Applications

A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction

Content Provider	MDPI
Author	Faassen, Elisabeth Antoniou, Maria Beekman-Lukassen, Wendy Blahova, Lucie Chernova, Ekaterina Christophoridis, Christophoros Combes, Audrey Edwards, Christine Fastner, Jutta Harmsen, Joop Hiskia, Anastasia Ilag, Leopold Kaloudis, Triantafyllos Lopicic, Srdjan Lürling, Miquel Mazur-Marzec, Hanna Meriluoto, Jussi Porojan, Cristina Viner-Mozzini, Yehudit Zguna, Nadezda
Abstract	Exposure to β-N-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer’s disease and Parkinson’s disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D3BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%–32%), implying that D3BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (<10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis.
File Size	1625088
File Format	PDF
e-ISSN	16603397
DOI	10.3390/md14030045
Journal	Marine Drugs
Issue Number	3
Volume Number	14
Language	English
Publisher Date	2016-02-29
Access Restriction	Open
Subject Keyword	β-N-methylamino-l-alanine (BMAA) 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) α,γ-diaminobutyric acid (DAB) cycad Daphnia magna hydrophilic interaction liquid chromatography (HILIC) Internal standard Liquid chromatography-tandem mass spectrometry (LC-MS/MS) N-(2-aminoethyl) glycine (AEG) phytoplankton seafood
Content Type	Text
Resource Type	Article

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New Typical Vector of Neurotoxin β-N-Methylamino-l-Alanine (BMAA) in the Marine Benthic Ecosystem

Beta-N-Methylamino-l-Alanine: LC-MS/MS Optimization, Screening of Cyanobacterial Strains and Occurrence in Shellfish from Thau, a French Mediterranean Lagoon

Detection of β-N-methylamino-l-alanine in postmortem olfactory bulbs of Alzheimer's disease patients using UHPLC-MS/MS: An autopsy case-series study.

Methods for the Chemical Analysis of β-N-Methylamino-L-A lanine: What Is Known and What Remains to Be Determined

Improved sensitivity using liquid chromatography mass spectrometry (LC-MS) for detection of propyl chloroformate derivatised β-N-methylamino-L-alanine (BMAA) in cyanobacteria

Occurrence of BMAA Isomers in Bloom-Impacted Lakes and Reservoirs of Brazil, Canada, France, Mexico, and the United Kingdom

Amino Acid Determination in Grape Juices and Wines by HPLC Using a Modification of the 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate (AQC) Method

Improved sensitivity using liquid chromatography mass spectrometry ( LC-MS ) for detection of propyl chloroformate derivatised β-N-methylamino-L-alanine ( BMAA ) in cyanobacteria

Simultaneous Determination of Glutamate, Glycine, and Alanine in Human Plasma Using Precolumn Derivatization with 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate and High-Performance Liquid Chromatography

A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction

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A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction