NDLI: Aptamer Based Microsphere Biosensor for Thrombin Detection

Content Provider	MDPI
Author	Zhu, Hongying Suter, Jonathan White, Ian Fan, Xudong
Abstract	We have developed an optical microsphere resonator biosensor using aptamer asreceptor for the measurement of the important biomolecule thrombin. The sphere surface ismodified with anti-thrombin aptamer, which has excellent binding affinity and selectivityfor thrombin. Binding of the thrombin at the sphere surface is monitored by the spectralposition of the microsphere’s whispering gallery mode resonances. A detection limit on theorder of 1 NIH Unit/mL is demonstrated. Control experiments with non-aptameroligonucleotide and BSA are also carried out to confirm the specific binding betweenaptamer and thrombin. We expect that this demonstration will lead to the development ofhighly sensitive biomarker sensors based on aptamer with lower cost and higher throughputthan current technology.
File Size	195584
Ending Page	795
Page Count	11
Starting Page	785
File Format	PDF
e-ISSN	14248220
DOI	10.3390/s6080785
Journal	Sensors
Issue Number	8
Volume Number	6
Language	English
Publisher Date	2006-08-16
Access Restriction	Open
Subject Keyword	Microsphere ring resonator whispering gallery mode aptamer thrombin
Content Type	Text
Resource Type	Article

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Aptamer Based Microsphere Biosensor for Thrombin Detection