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| Content Provider | MDPI |
|---|---|
| Author | Ohyashiki, Kazuma Umezu, Tomohiro Katagiri, Seiichiro Kobayashi, Chiaki Azuma, Kenko Tauchi, Tetsuzo Okabe, Seiichi Fukuoka, Yutaka Ohyashiki, Junko |
| Abstract | Approximately 40% of chronic myeloid leukemia (CML) patients who discontinue imatinib (IM) therapy maintain undetectable minimal residual disease (UMRD) for more than one year (stopping IM (STOP-IM)). To determine a possible biomarker for STOP-IM CML, we examined plasma miRNA expression in CML patients who were able to discontinue IM. We first screened candidate miRNAs in unselected STOP-IM patients, who had sustained UMRD after discontinuing IM for more than six months, in comparison with healthy volunteers, by using a TaqMan low-density array for plasma or exosomes. Exosomal miR-215 and plasma miR-215 were downregulated in the STOP-IM group compared to the control, indicating that the biological relevance of the plasma miR-215 level is equivalent to that of the exosomal level. Next, we performed real-time quantitative RT-PCR in 20 STOP-IM patients, 32 patients with UMRD on continued IM therapy (IM group) and 28 healthy volunteers. The plasma miRNA-215 level was significantly downregulated in the STOP-IM group (p < 0.0001); we determined the cut-off level and divided the IM group patients into two groups according to whether the plasma miR-215 was downregulated or not. The IM group patients with a low plasma miR-215 level had a significantly higher total IM intake, compared to the patients with elevated miR-215 levels (p = 0.0229). Functional annotation of miR-215 target genes estimated by the Database for Annotation, Visualization and Integrated Discovery (DAVID) bioinformatic tools involved cell cycle, mitosis, DNA repair and cell cycle checkpoint. Our study suggests a possible role of miR-215 in successful IM discontinuation. |
| File Size | 1474560 |
| File Format | |
| e-ISSN | 14220067 |
| DOI | 10.3390/ijms17040570 |
| Journal | International Journal of Molecular Sciences |
| Issue Number | 4 |
| Volume Number | 17 |
| Language | English |
| Publisher Date | 2016-04-15 |
| Access Restriction | Open |
| Subject Keyword | plasma miR-215 chronic myeloid leukemia imatinib discontinuation |
| Content Type | Text |
| Resource Type | Article |
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