NDLI: Characterization and Bioactivity of Polysaccharides Obtained from Pine Cones of Pinus koraiensis by Graded Ethanol Precipitation

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Characterization and Bioactivity of Polysaccharides Obtained from Pine Cones of Pinus koraiensis by Graded Ethanol Precipitation

Content Provider	MDPI
Author	Zou, Pan Yang, Xin Huang, Wei-Wei Zhao, Hai-Tian Wang, Jing Xu, Ren-Bo Hu, Xing-Long Shen, Si-Yan Qin, Di
Abstract	Pinus koraiensis polysaccharides (PKP) were extracted by hot water from P. koraiensis pine cones. Five polysaccharide fractions named PKP-A, PKP-B, PKP-C, PKP-D and PKP-E were successfully separated at final ethanol concentrations of 30%, 50%, 60%, 70% and 80%, respectively. HPLC, FT-IR, GC-MS and automatic amino-acid analysis were applied to investigate their chemical characteristics. Monosaccharide component analysis indicated that the five fractions were all composed of d-ribose, l-rhamnose, l-arabinose, d-xylose, d-mannose, d-glucose and d-galactose, but their molar ratios were quite different. HPLC results revealed that the polysaccharides precipitated by higher concentrations of ethanol solution had lower molecular masses. Moreover, the antioxidant activities of the five fractions were studied on the basis of hydroxyl radical and ABTS radical scavenging tests. The five graded polysaccharide fractions exhibited good inhibitory power, and MTT tests in vitro showed the IC50 of PKP-A and PKP-E were 1,072.5 and 2,070.0 μg·mL−1, respectively. These results demonstrated that the PKP could be a potential source of natural antioxidants or dietary supplements.
File Size	290816
Ending Page	9948
Page Count	16
Starting Page	9933
File Format	PDF
e-ISSN	14203049
DOI	10.3390/molecules18089933
Journal	Molecules
Issue Number	8
Volume Number	18
Language	English
Publisher Date	2013-08-19
Access Restriction	Open
Subject Keyword	pine cones Pinus koraiensis polysaccharides graded ethanol precipitation chemical characterization bioactivity
Content Type	Text
Resource Type	Article

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