NDLI: Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates

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Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates

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Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates

Content Provider	MDPI
Author	Recek, Nina Jaganjac, Morana Kolar, Metod Milkovic, Lidija Mozetič, Miran Stana-Kleinschek, Karin Vesel, Alenka
Abstract	Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.
File Size	2501632
Ending Page	12463
Page Count	23
Starting Page	12441
File Format	PDF
e-ISSN	14203049
DOI	10.3390/molecules181012441
Journal	Molecules
Issue Number	10
Volume Number	18
Language	English
Publisher Date	2013-10-10
Access Restriction	Open
Subject Keyword	oxygen and fluorine plasma treatment polymer surface modification protein adsorption cell adhesion quartz crystal microbalance (QCM)
Content Type	Text
Resource Type	Article

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