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Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract.
| Content Provider | LitCovid |
|---|---|
| Author | Sun, Fu Ganguli, Anurup Nguyen, Judy Brisbin, Ryan Shanmugam, Krithika Hirschberg, David L Wheeler, Matthew B Bashir, Rashid Nash, David M Cunningham, Brian T |
| Abstract | Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in approximately 30 minutes. |
| ISSN | 14730197 14730189 |
| DOI | 10.1039/d0lc00304b |
| Journal | Lab Chip |
| PubMed reference number | 32334422 |
| Language | English |
| Publisher Date | 2020-04-26 |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Chemistry Biochemistry Bioengineering Biomedical Engineering |
