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Molecular marker assisted introgression of null allele of kunitz trypsin inhibitor (ti) in soybean (Glycine max L.)
| Content Provider | KrishiKosh-Indian National Agricultural Research System |
|---|---|
| Advisor | Talukdar, A. |
| Researcher | Shivakumar, M. |
| Abstract | Kunitz trypsin inhibitor (KTI), a major anti-nutritional factor present in soybean seed, is a major bottleneck in industrial use of soybean and public acceptance of soybean food products. Biochemical screening was carried out for analyzing trypsin inhibitor contents in 145 genotypes comprising exotic collections and released varieties. The content of trypsin inhibitor ranged from 14.15 to 186.27 mg g-1 of seed meal. Lowest level of trypsin inhibitor content was observed in genotype PI542044, (14.15 mg g-1 seed meal). Popular Indian soybean varieties viz., JS335, JS9305, NRC37, NRC7, PK1225, DS9712, JS9752, and DS9814 contained higher level of trypsin inhibitor. Heat treatment could reduce trypsin inhibitor activity partially leaving about 20% as residual activity. The study indicated the need for other ways like site directed mutagenesis to create genotypes with reduced KTI for use in breeding program. |
| File Size | 4637094 |
| File Format | |
| Language | English |
| Publisher | IARI |
| Publisher Place | New Delhi |
| Access Restriction | Open |
| Subject Keyword | Soybean Kunitz Trypsin Inhibitor Biochemical Screening Residual Activity and Ti Locus |
| Content Type | Text |
| Educational Degree | Doctor of Philosophy (Ph.D.) |
| Resource Type | Thesis |