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Cu-Chi-Tri: A triple combination for the management of late blight disease of potato (Solanum tuberosum L.)
| Content Provider | KrishiKosh-Indian National Agricultural Research System |
|---|---|
| Advisor | Kumar, J. |
| Researcher | Sajeesh, P. K. |
| Abstract | Potato (Solanum tubersum) is world's fourth-largest food crop, following rice, wheat and maize. Late blight of potato caused by Phytophthora infestans is probably the most destructive plant disease. Under favourable environmental conditions the entire crop could be destroyed within a week after appearance of the disease. Disease management with copperbased fungicides still remains the largely and widely adopted practice but risks several components of agro-ecosystem. Therefore, an alternative approach that brings about reduction in the usage of copper and offer similar level of crop protection is the need. The knowledgebased triple combination of copper (Cu), plant strengthener; chitosan (Chi) and copper tolerant and chitosanolytic Trichoderma (Tri) a new generation plant protectant was found to significantly reduce the late blight disease of potato at field level. In the present investigation, chitosan (500ppm) in combination with reduced dose of copper (500ppm) and Trichoderma (1%) was used to manage this disease in field trials conducted over multiple years in plains and hills. All the treatments were used as seed and foliar applications. Among different treatments tested during 2013-15, minimum disease severity (1.0- 5.0 %) was observed with the standard fungicide Mancozeb (2000ppm) and was at par with triple combination (2.0 -7.0%) but was significantly different from CuOH (1000ppm) and check at 85 days after planting. The decrease in disease severity translated into increase in yield of potato. Maximum potato tuber yield was obtained with Mancozeb (2000ppm) and was at par with triple combination but was significantly different from CuOH (1000ppm) and check. The mechanism of disease reduction through triple combination was analysed and it was mainly due to the induced resistance in host plant. The induction of defense related H2O2, SOD, PAL, PPO and PO as well as the secondary metabolites like phenol and proline accumulation was maximum during 24 to 48 hr of post inoculation of P. infestans compared to control as well as CuOH alone. The real-time expression analysis of defence genes viz., St NPR1, HSP, ABA, SOD and ZFR revealed that the fold increase was more in triple combination treatment as compared to CuOH alone and control at 24 hr post inoculation with P. infestans. It was observed that the triple combination, besides giving significant disease control, was also quite effective in increasing tuber quality in terms of nutrition. Significantly higher protein content (3-3.5%) of the potato was observed in triple combination as compared to Mancozeb (2%) and control (1.8%). Accumulation of patatin (40-43KDa), a storage and defence protein was also more in tubers harvested from triple combination as compared to Mancozeb and control. However, in case of total soluble sugar, reducing sugar and non-reducing sugar content no significant differences were observed among triple combination, Mancozeb and control. The result revealed that triple combination so designed could be a viable alternative to chemical fungicides for disease management as well as for increasing the tuber yield and quality of potato. |
| File Size | 12922953 |
| File Format | |
| Language | English |
| Publisher | G.B. Pant University of Agriculture and Technology |
| Publisher Place | Pantnagar |
| Access Restriction | Open |
| Subject Keyword | Copper Chitosan Trichoderma Disease Control Blight Potatoes Solanum Tuberosum Fungal Diseases Biological Control |
| Content Type | Text |
| Resource Type | Thesis |
