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Effect of Root rot of Safed musli (Chlorophytum borivilianum) on saponin content and its Management
| Content Provider | KrishiKosh-Indian National Agricultural Research System |
|---|---|
| Advisor | Sharma, S. S. |
| Researcher | Verma, Kalp |
| Abstract | During growing season of Safed musli (Chlorophytum borivilianum) surveys were carried out to collect root rot infected samples and rhizosphere soil from healthy roots from diverse locations of zone IVa and V of Rajasthan viz., Jhalawar, Sirohi district and Jhadol tehsil of Udaipur district. Out of survey Fusarium solani (ITCC-6569) and Rhizoctonia solani (ITCC-6570) were isolated from diseased roots. Pathogenicity of these cultures was established by inoculating the pathogens developed on corn meal and sand (1:1) medium, which was mixed in pots containing sterilized soil with healthy roots/fingers. Two resident isolates of Trichoderma (ITCC- 6571 and ITCC-6572) were also recovered from rhizosphere soil of healthy roots. All cultures were purified using hyphal tip culture technique. The cultures were deposited to ITCC and identity was confirmed. Effect of Root rot on saponin content was estimated with biochemical analysis of diseased and healthy dried roots. It was found that infected roots contain more saponin content (0.71g) as compared to healthy one (0.64g). Studies were conducted in vitro to assess effective conc. of bavistin and four isolates of Trichoderma alone or in combinations for suppression of mycelial growth of both root rot pathogens. Studies revealed that 0.05 per cent conc. caused complete inhibition of both root pathogens. One of the resident isolate of Trichoderma (ITCC-6572) was found extremely antagonistic to both root rot pathogens in contrast to other isolates. Combination of this potent isolate with 0.05 per cent conc. of bavistin caused complete inhibition of both root rot pathogens. Although, it was observed that all conc. of bavistin except 0.01 per cent inhibited this potent isolate of Trichoderma. This shows that combination of bioagent and bavistin can not be used. However, lower conc of bavistin (0.01 or less than 0.01 per cent) with bioagent may work well. |
| Page Count | 41 |
| File Size | 210932 |
| File Format | |
| Language | English |
| Publisher | MPUAT |
| Publisher Place | Udaipur |
| Access Restriction | Open |
| Subject Keyword | Root Rot Chlorophytum Borivilianum Saponin Management Effect of Root Rot of Safed Musli (chlorophytum Borivilianum) On Saponin Content and Its Management Plant Pathology |
| Content Type | Text |
| Educational Degree | Master of Science (M.Sc.) |
| Resource Type | Thesis |