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MOLECULAR MAPPING OF CHARCOAL ROT RESISTANCE IN SOYBEAN (Glycine max L. MERR.)
| Content Provider | KrishiKosh-Indian National Agricultural Research System |
|---|---|
| Advisor | Talukdar, Akshay |
| Researcher | Samrat, Gowda D. S. |
| Abstract | Soybean Glycine max L. Merr. is the most important oilseed crop in India. It contains more than 40% protein and 20% high quality oil along with important minerals and nutrition elements. It has got numerous applications as food, feed and fuel and in health and pharmaceutical industries. The area and production of soybean in India has increased rapidly; however average productivity (~1.3 t/ha) is still below the world average (~2.5 t/ha). Among various factors of low productivity, charcoal rot disease caused by fungus Macrophomina phaseolina is the most damaging one. The disease can cause more than 70% losses in severe cases. Cultural and chemical control of the disease is not remunerative; hence genetic resistance has been strongly promoted. In this study, a set of 100 soybean-germplasm lines were screened in field, pots, blotter-paper and in vitro, and identified seven genotypes viz., DS9712, DS9814, JS335, PK564, EC439619, EC439618 and EC44303 as resistant to charcoal rot disease. Resistant genotype JS335 was crossed with a highly susceptible genotype MACS985 to produce a mapping population. Analysis of 400 SSR markers indicated that the level of polymorphism between the parents was 29.25%. Segregation studies in 120 F2 plants indicated that charcoal rot resistance in JS335 is governed by polygenes. Mapping studies in the F2 plants with 117 SSR markers detected 4 QTL for charcoal rot resistance. Two QTL viz., qCr-2 and qCr-6 had LOD score 3.1, and were mapped in chromosomes 2 and 6, respectively. The QTL qCr-2 was flanked by markers satt542 and satt266 while qCr-6 was flanked by satt640 and satt460. Other two QTL viz., qCr-16 and qCr-3 had LOD score 2.4 and 2.5, and were mapped in chromosomes 16 and 3, respectively. Phenotypic variance explained by the QTL ranged from 10-15%. Markers flanking the QTL were validated in unrelated genotypes. The resistant genotypes identified in this study will be suitable donor for charcoal rot resistance. Testing of the genotypes against other isolates of M phaseolina, and validation of the markers in larger segregating population will affirm its applicability in marker-assisted selection (MAS) for charcoal resistance in soybean. |
| File Size | 2473551 |
| File Format | |
| Language | English |
| Publisher | DIVISION OF GENETICS INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI |
| Access Restriction | Open |
| Subject Keyword | Molecular Mapping of Charcoal Rot Resistance In Soybean (glycine Max L. Merr.) Genetics and Plant Breeding |
| Content Type | Text |
| Educational Degree | Doctor of Philosophy (Ph.D.) |
| Resource Type | Thesis |
