NDLI: Spatially dependent alkyl quinolone signaling responses to antibiotics in Pseudomonas aeruginosa swarms

Content Provider	Journal of Biological Chemistry (JBC)
Author	Morales-Soto, Nydia Dunham, Sage J. B. Baig, Nameera F. Ellis, Joseph F. Madukoma, Chinedu S. Bohn, Paul W. Sweedler, Jonathan V. Shrout, Joshua D.
Abstract	There is a general lack of understanding about how communities of bacteria respond to exogenous toxins such as antibiotics. Most of our understanding of community-level stress responses comes from the study of stationary biofilm communities. Although several community behaviors and production of specific biomolecules affecting biofilm development and associated behavior have been described for Pseudomonas aeruginosa and other bacteria, we have little appreciation for the production and dispersal of secreted metabolites within the 2D and 3D spaces they occupy as they colonize, spread, and grow on surfaces. Here we specifically studied the phenotypic responses and spatial variability of alkyl quinolones, including the Pseudomonas quinolone signal (PQS) and members of the alkyl hydroxyquinoline (AQNO) subclass, in P. aeruginosa plate-assay swarming communities. We found that PQS production was not a universal signaling response to antibiotics, as tobramycin elicited an alkyl quinolone response, whereas carbenicillin did not. We also found that PQS and AQNO profiles in response to tobramycin were markedly distinct and influenced these swarms on different spatial scales. At some tobramycin exposures, P. aeruginosa swarms produced alkyl quinolones in the range of 150 μm PQS and 400 μm AQNO that accumulated as aggregates. Our collective findings show that the distribution of alkyl quinolones can vary by several orders of magnitude within the same swarming community. More notably, our results suggest that multiple intercellular signals acting on different spatial scales can be triggered by one common cue.
Related Links	http://www.jbc.org/content/293/24/9544.abstract
Ending Page	9552
Starting Page	9544
Page Count	9
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	24
Volume Number	293
DOI	10.1074/jbc.RA118.002605
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2018-06-15
Access Restriction	Open
Subject Keyword	Pseudomonas aeruginosa (P. aeruginosa) Antibiotics Quorum sensing Raman spectroscopy Mass spectrometry (MS) Biofilm N-oxide PQS Quinolone Secondary ion mass spectrometry Swarming Microbiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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