NDLI: A Self-compartmentalizing Hexamer Serine Protease from Pyrococcus Horikoshii SUBSTRATE SELECTION ACHIEVED THROUGH MULTIMERIZATION

Content Provider	Journal of Biological Chemistry (JBC)
Author	Menyhárd, Dóra K. Kiss-Szemán, Anna Tichy-Rács, Éva Hornung, Balázs Rádi, Krisztina Szeltner, Zoltán Domokos, Klarissza Szamosi, Ilona Náray-Szabó, Gábor Polgár, László Harmat, Veronika
Abstract	Oligopeptidases impose a size limitation on their substrates, the mechanism of which has long been under debate. Here we present the structure of a hexameric serine protease, an oligopeptidase from Pyrococcus horikoshii (PhAAP), revealing a complex, self-compartmentalized inner space, where substrates may access the monomer active sites passing through a double-gated “check-in” system, first passing through a pore on the hexamer surface and then turning to enter through an even smaller opening at the monomers' domain interface. This substrate screening strategy is unique within the family. We found that among oligopeptidases, a residue of the catalytic apparatus is positioned near an amylogenic β-edge, which needs to be protected to prevent aggregation, and we found that different oligopeptidases use different strategies to achieve such an end. We propose that self-assembly within the family results in characteristically different substrate selection mechanisms coupled to different multimerization states.
Related Links	http://www.jbc.org/content/288/24/17884.abstract
Ending Page	17894
Starting Page	17884
Page Count	11
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	24
Volume Number	288
DOI	10.1074/jbc.M113.451534
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2013-06-14
Access Restriction	Open
Subject Keyword	Aggregation Peptide Interactions Protein Self-assembly Serine Protease X-ray Crystallography Acylaminoacyl Peptidase Amylogenic β-Strand Self-compartmentalization Substrate Size Selection Protein Structure and Folding
Alternative Title	A Self-compartmentalizing Hexamer Serine Protease from Pyrococcus Horikoshii
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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