NDLI: Mgm101 Is a Rad52-related Protein Required for Mitochondrial DNA Recombination

Content Provider	Journal of Biological Chemistry (JBC)
Author	Mbantenkhu, MacMillan Wang, Xiaowen Nardozzi, Jonathan D. Wilkens, Stephan Hoffman, Elizabeth Patel, Anamika Cosgrove, Michael S. Chen, Xin Jie
Abstract	Homologous recombination is a conserved molecular process that has primarily evolved for the repair of double-stranded DNA breaks and stalled replication forks. However, the recombination machinery in mitochondria is poorly understood. Here, we show that the yeast mitochondrial nucleoid protein, Mgm101, is related to the Rad52-type recombination proteins that are widespread in organisms from bacteriophage to humans. Mgm101 is required for repeat-mediated recombination and suppression of mtDNA fragmentation in vivo. It preferentially binds to single-stranded DNA and catalyzes the annealing of ssDNA precomplexed with the mitochondrial ssDNA-binding protein, Rim1. Transmission electron microscopy showed that Mgm101 forms large oligomeric rings of ∼14-fold symmetry and highly compressed helical filaments. Specific mutations affecting ring formation reduce protein stability in vitro. The data suggest that the ring structure may provide a scaffold for stabilization of Mgm101 by preventing the aggregation of the otherwise unstable monomeric conformation. Upon binding to ssDNA, Mgm101 is remobilized from the rings to form distinct nucleoprotein filaments. These studies reveal a recombination protein of likely bacteriophage origin in mitochondria and support the notion that recombination is indispensable for mtDNA integrity.
Related Links	http://www.jbc.org/content/286/49/42360.abstract
Ending Page	42370
Starting Page	42360
Page Count	11
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	49
Volume Number	286
DOI	10.1074/jbc.M111.307512
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2011-12-09
Access Restriction	Open
Subject Keyword	Genetics Mitochondria Mitochondrial DNA (mtDNA) Yeast Yeast Genetics Bioenergetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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