NDLI: InvA Protein Is a Nudix Hydrolase Required for Infection by Pathogenic Leptospira in Cell Lines and Animals

Content Provider	Journal of Biological Chemistry (JBC)
Author	Luo, Yihui Liu, Yan Sun, Dexter Ojcius, David M. Zhao, Jinfang Lin, Xuai Wu, Dong Zhang, Rongguang Chen, Ming Li, Lanjuan Yan, Jie
Abstract	Leptospirosis caused by pathogenic species of the genus Leptospira is a re-emerging zoonotic disease, which affects a wide variety of host species and is transmitted by contaminated water. The genomes of several pathogenic Leptospira species contain a gene named invA, which contains a Nudix domain. However, the function of this gene has never been characterized. Here, we demonstrated that the invA gene was highly conserved in protein sequence and present in all tested pathogenic Leptospira species. The recombinant InvA protein of pathogenic L. interrogans strain Lai hydrolyzed several specific dinucleoside oligophosphate substrates, reflecting the enzymatic activity of Nudix in Leptospira species. Pathogenic leptospires did not express this protein in media but temporarily expressed it at early stages (within 60 min) of infection of macrophages and nephric epithelial cells. Comparing with the wild type, the invA-deficient mutant displayed much lower infectivity and a significantly reduced survival rate in macrophages and nephric epithelial cells. Moreover, the invA-deficient leptospires presented an attenuated virulence in hamsters, caused mild histopathological damage, and were transmitted in lower numbers in the urine, compared with the wild-type strain. The invA revertant, made by complementing the invA-deficient mutant with the invA gene, reacquired virulence similar to the wild type in vitro and in vivo. The LD50 in hamsters was 1000-fold higher for the invA-deficient mutant than for the invA revertant and wild type. These results demonstrate that the InvA protein is a Nudix hydrolase, and the invA gene is essential for virulence in pathogenic Leptospira species.
Related Links	http://www.jbc.org/content/286/42/36852.abstract
Ending Page	36863
Starting Page	36852
Page Count	12
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	42
Volume Number	286
DOI	10.1074/jbc.M111.219931
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2011-10-21
Access Restriction	Open
Subject Keyword	Bacteria Bacterial Toxins Cell-Cell Interaction Gene Knockout Pathogen-associated Molecular Pattern (PAMP) Nudix Hydrolase Leptospire Pathogenesis Survival Virulence Molecular Bases of Disease
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Retraction. InvA protein is a Nudix hydrolase required for infection by pathogenic Leptospira in cell lines and animals.

InvA Protein Is a Nudix Hydrolase Required for Infection by Pathogenic Leptospira in Cell Lines and Animals*

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