NDLI: Thiolated tRNAs of Trypanosoma brucei Are Imported into Mitochondria and Dethiolated after Import

Content Provider	Journal of Biological Chemistry (JBC)
Author	Bruske, Ellen Inga Sendfeld, Franziska Schneider, André
Abstract	All mitochondrial tRNAs in Trypanosoma brucei derive from cytosolic tRNAs that are in part imported into mitochondria. Some trypanosomal tRNAs are thiolated in a compartment-specific manner. We have identified three proteins required for the thio modification of cytosolic tRNAGln, tRNAGlu, and tRNALys. RNA interference-mediated ablation of these proteins results in the cytosolic accumulation non-thio-modified tRNAs but does not increase their import. Moreover, in vitro import experiments showed that both thio-modified and non-thio-modified tRNAGlu can efficiently be imported into mitochondria. These results indicate that unlike previously suggested the cytosol-specific thio modifications do not function as antideterminants for mitochondrial tRNA import. Consistent with these results we showed by using inducible expression of a tagged tRNAGlu that it is mainly the thiolated form that is imported in vivo. Unexpectedly, the imported tRNA becomes dethiolated after import, which explains why the non-thiolated form is enriched in mitochondria. Finally, we have identified two genes required for thiolation of imported tRNATrp whose wobble nucleotide is subject to mitochondrial C to U editing. Interestingly, down-regulation of thiolation resulted in an increase of edited tRNATrp but did not affect growth.
Related Links	http://www.jbc.org/content/284/52/36491.abstract
Ending Page	36499
Starting Page	36491
Page Count	9
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	52
Volume Number	284
DOI	10.1074/jbc.M109.064527
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2009-12-25
Access Restriction	Open
Subject Keyword	RNA: Processing and Catalysis
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Thiolated tRNAs of Trypanosoma brucei Are Imported into Mitochondria and Dethiolated after Import*

Thiolated tRNAs of Trypanosoma brucei are imported into mitochondria and dethiolated after import.

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A nuclear encoded tRNA of Trypanosoma brucei is imported into mitochondria.

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Thiolated tRNAs of Trypanosoma brucei Are Imported into Mitochondria and Dethiolated after Import

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Thiolated tRNAs of Trypanosoma brucei Are Imported into Mitochondria and Dethiolated after Import*

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