NDLI: The Role of Lhca Complexes in the Supramolecular Organization of Higher Plant Photosystem I

Content Provider	Journal of Biological Chemistry (JBC)
Author	Wientjes, Emilie Oostergetel, Gert T. Jansson, Stefan Boekema, Egbert J. Croce, Roberta
Abstract	In this work, Photosystem I supercomplexes have been purified from Lhca-deficient lines of Arabidopsis thaliana using a mild detergent treatment that does not induce loss of outer antennas. The complexes have been studied by integrating biochemical analysis with electron microscopy. This allows the direct correlation of changes in protein content with changes in supramolecular structure of Photosystem I to get information about the position of the individual Lhca subunits, the association of the antenna to the core, and the influence of the individual subunits on the stability of the system. Photosystem I complexes with only two or three antenna complexes were purified, showing that the binding of Lhca1/4 and Lhca2/3 dimers to the core is not interdependent, although weak binding of Lhca2/3 to the core is stabilized by the presence of Lhca4. Moreover, Lhca2 and Lhca4 can be associated with the core in the absence of their “dimeric partners.” The structure of Photosystem I is very rigid, and the absence of one antenna complex leaves a “hole” in the structure that cannot be filled by other Lhcas, clearly indicating that the docking sites for the individual subunits are highly specific. There is, however, an exception to the rule: Lhca5 can substitute for Lhca4, yielding highly stable PSI supercomplexes with a supramolecular organization identical to the WT.
Related Links	http://www.jbc.org/content/284/12/7803.abstract
Ending Page	7810
Starting Page	7803
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	12
Volume Number	284
DOI	10.1074/jbc.M808395200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2009-03-20
Access Restriction	Open
Subject Keyword	Metabolism and Bioenergetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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