NDLI: Transcriptional Regulation of Acetylcholinesterase-associated Collagen ColQ DIFFERENTIAL EXPRESSION IN FAST AND SLOW TWITCH MUSCLE FIBERS IS DRIVEN BY DISTINCT PROMOTERS

Content Provider	Journal of Biological Chemistry (JBC)
Author	Lee, Henry H. C. Choi, Roy C. Y. Ting, Annie K. L. Siow, Nina L. Jiang, Joy X. S. Massoulié, Jean Tsim, Karl W. K.
Abstract	The presence of a collagenous protein (ColQ) characterizes the collagen-tailed forms of acetylcholinesterase and butyrylcholinesterase at vertebrate neuromuscular junctions which is tethered in the synaptic basal lamina. ColQ subunits, differing mostly by their signal sequences, are encoded by transcripts ColQ-1 and ColQ-1a, which are differentially expressed in slow and fast twitch muscles in mammals. Two distinct promoters, pColQ-1 and pColQ-1a, were isolated from the upstream sequences of human COLQ gene; they showed muscle-specific expression and were activated by myogenic transcriptional elements in cultured myotubes. After in vivo DNA transfection, pColQ-1 showed strong activity in slow twitch muscle (e.g. soleus), whereas pColQ-1a was preferably expressed in fast twitch muscle (e.g. tibialis). Mutation analysis of the ColQ promoters suggested that the muscle fiber type-specific expression pattern of ColQ transcripts were regulated by a slow upsteam regulatory element (SURE) and a fast intronic regulatory element (FIRE). These regulatory elements were responsive to a calcium ionophore and to calcineurin inhibition by cyclosporine A. The slow fiber type-specific expression of ColQ-1 was abolished by the mutation of an NFAT element in pColQ-1. Moreover, both the ColQ promoters contained N-box element that was responsible for the synapse-specific expression of ColQ transcripts. These results explain the specific expression patterns of collagen-tailed acetylcholinesterase in slow and fast muscle fibers.
Related Links	http://www.jbc.org/content/279/26/27098.abstract
Ending Page	27107
Starting Page	27098
Page Count	10
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	26
Volume Number	279
DOI	10.1074/jbc.M402596200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2004-06-25
Access Restriction	Open
Subject Keyword	Genes: Structure and Regulation
Alternative Title	Transcriptional Regulation of Acetylcholinesterase-associated Collagen ColQ
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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