NDLI: Mechanism Underlying the Inner Membrane Retention of Escherichia coli Lipoproteins Caused by Lol Avoidance Signals

Content Provider	Journal of Biological Chemistry (JBC)
Author	Hara, Takashi Matsuyama, Shin-ichi Tokuda, Hajime
Abstract	Escherichia coli lipoproteins are localized to either the inner or outer membrane depending on the residue at position 2. The inner membrane retention signal, Asp at position 2 in combination with certain residues at position 3, functions as a Lol avoidance signal, i.e. the signal inhibits the recognition of lipoproteins by LolCDE that releases lipoproteins from the inner membrane. To understand the role of the residue at position 2, outer membrane-specific lipoproteins with Cys at position 2 were subjected to chemical modification followed by the release reaction in reconstituted proteoliposomes. Sulfhydryl-specific introduction of nonprotein molecules or a negative charge to Cys did not inhibit the LolCDE-dependent release. In contrast, oxidation of Cys to cysteic acid resulted in generation of the Lol avoidance signal, indicating that the Lol avoidance signal requires a critical length of negative charge at the second residue. Furthermore, not only modification of the carboxylic acid of Asp at position 2 but also that of the amine of phosphatidylethanolamine abolished the Lol avoidance function. Based on these results, the Lol avoidance mechanism is discussed.
Related Links	http://www.jbc.org/content/278/41/40408.abstract
Ending Page	40414
Starting Page	40408
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	41
Volume Number	278
DOI	10.1074/jbc.M307836200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2003-10-10
Access Restriction	Open
Subject Keyword	Membrane Transport, Structure, Function, and Biogenesis
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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