NDLI: trans-Targeting of the Phage Mu Repressor Is Promoted by Conformational Changes That Expose Its ClpX Recognition Determinant

Content Provider	Journal of Biological Chemistry (JBC)
Author	Marshall-Batty, Kimberly R. Nakai, Hiroshi
Abstract	Dominant negative forms of the phage Mu repressor, including the mutant Vir repressors, are not only rapidly degraded by the ClpXP protease but also promote degradation of the unmodified, wild-type repressor. This trans-targeting of the wild-type repressor depends upon a determinant within its C-terminal domain, which is needed for recognition by ClpX. An environmentally sensitive fluorescent probe (2-(4′-maleimidylanilino)naphthalene-6-sulfonic acid (MIANS)) attached to the C terminus of the full-length repressor indicated that Vir induces the movement of this domain into a more exposed configuration. Vir also promoted attachment of MIANS to the C terminus of the repressor at an accelerated rate, and it greatly increased the rate of phosphorylation of a cAMP-dependent protein kinase motif attached to the repressor C terminus. While an excess of Vir was needed to promote repressor phosphorylation at maximal rates, the presence of ClpX could increase phosphorylation rates at lower Vir levels.trans-Targeting of the Mu repressor is therefore promoted by exposing its ClpX recognition determinant, and the action of ClpX can assist Vir in exposing these determinants.
Related Links	http://www.jbc.org/content/278/3/1612.abstract
Ending Page	1617
Starting Page	1612
Page Count	6
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	3
Volume Number	278
DOI	10.1074/jbc.M209352200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2003-01-17
Access Restriction	Open
Subject Keyword	Repressor encoded by a virulent mutant of Mu (Vir) Vir repressor with a C17A alteration and a cysteine added at the C terminus (VirC192) Cysteine-less Vir repressor having a C17A alteration (VirC17A) C-terminal domain (CTD) DNA binding domain (DBD) 2-(4′-maleimidylanilino)naphthalene-6-sulfonic acid (MIANS) Wild-type Mu repressor (Rep) Repressor truncated by 18 residues at the C-terminal end (RepΔ18) Repressor with a C17A alteration and a cysteine residue added at the C terminus (RepC197) Cysteine-less repressor having a C17A alteration (RepC17A) Repressor with a kinase motif at the N terminus (RepNK) Repressor with a kinase motif at the C terminus (RepCK) Catalytic subunit of the cAMP-dependent protein kinase (PKA) PROTEIN SYNTHESIS POST-TRANSLATION MODIFICATION AND DEGRADATION
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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