NDLI: Versican Interacts with Fibrillin-1 and Links Extracellular Microfibrils to Other Connective Tissue Networks

Content Provider	Journal of Biological Chemistry (JBC)
Author	Isogai, Zenzo Aspberg, Anders Keene, Douglas R. Ono, Robert N. Reinhardt, Dieter P. Sakai, Lynn Y.
Abstract	Fibrillin-containing microfibrils are polymeric structures that are difficult to extract from connective tissues. Proteolytic digestion of tissues has been utilized to release microfibrils for study. Few of the molecules that connect microfibrils to other elements in the matrix have been identified. In this study, electron microscopic immunolocalization of anti-versican antibodies in tissues and in extracted microfibrils demonstrated that the C-terminal region of versican is found associated with fibrillin microfibrils. Extraction of microfibrils followed by treatment of microfibrils under dissociating conditions suggested that the versican C terminus is covalently bound to microfibrils. Binding assays using recombinant fibrillin-1 polypeptides and recombinant lectican lectin domains indicated that the versican lectin domain binds to specific fibrillin-1 polypeptides. The versican lectin domain also bound to molecules comigrating with authentic fibrillin-1 monomers in an assay using cell culture medium. In assays using microfibrils, the versican lectin domain demonstrated preferential binding compared with other lecticans. Binding was calcium-dependent. The binding site for versican in microfibrils is most likely within a region of fibrillin-1 between calcium-binding epidermal growth factor-like domains 11 and 21. Human mutations in this region can result in severe forms of the Marfan syndrome (“neonatal” Marfan syndrome). The connection between versican and fibrillin microfibrils may be functionally significant, particularly in cardiovascular tissues.
Related Links	http://www.jbc.org/content/277/6/4565.abstract
Ending Page	4572
Starting Page	4565
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	6
Volume Number	277
DOI	10.1074/jbc.M110583200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2002-02-08
Access Restriction	Open
Subject Keyword	Extracellular matrix (ECM) Epidermal growth factor (EGF) Monoclonal antibody (mAb) Tris-buffered saline (TBS) Calcium-binding epidermal growth factor (cbEGF) GLYCOBIOLOGY AND EXTRACELLULAR MATRICES
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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