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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Guo, Qing Gong, Qingguo Tong, Ka-Lok Vestergaard, Bente Costa, Annie Desgres, Jean Wong, Mansim Grosjean, Henri Zhu, Guang Wong, J. Tze-Fei Xue, Hong |
| Abstract | To study the recognition by tryptophanyl-tRNA synthetase (TrpRS) of tRNATrp discriminator base, mutations were introduced into the discriminator base of Bacillus subtilis, Archeoglobus fulgidus, and bovine tRNATrp, representing the three biological domains. WhenB. subtilis, A. fulgidus, and human TrpRS were used to acylate these tRNATrp, two distinct preference profiles regarding the discriminator base of different tRNATrp substrates were found: G>A>U>C for B. subtilis TrpRS, and A>C>U>G for A. fulgidus and human TrpRS. The preference for G73 in tRNATrp by bacterial TrpRS is much stronger than the modest preferences for A73 by the archaeal and eukaryotic TrpRS. Cross-species reactivities between TrpRS and tRNATrp from the three domains were in accordance with the view that the evolutionary position of archaea is intermediate between those of eukarya and bacteria. NMR spectroscopy revealed that mutation of A73 to G73 in bovine tRNATrp elicited a conformational alteration in the G1-C72 base pair. Mutation of G1-C72 to A1-U72 or disruption of the G1-C72 base pair also caused reduction of Trp-tRNATrp formation. These observations identify a tRNATrp structural region near the end of acceptor stem comprising A73 and G1-C72 as a crucial domain required for effective recognition by human TrpRS. |
| Related Links | http://www.jbc.org/content/277/16/14343.abstract |
| Ending Page | 14349 |
| Starting Page | 14343 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 16 |
| Volume Number | 277 |
| DOI | 10.1074/jbc.M111745200 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2002-04-19 |
| Access Restriction | Open |
| Subject Keyword | Tryptophanyl-tRNA synthetase (TrpRS) TRNA for Trp (tRNATrp) Isopropyl-β-d-thiogalactopyranoside (IPTG) Heteronuclear single-quantum correlation (HSQC) RNA: STRUCTURE METABOLISM AND CATALYSIS |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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