NDLI: NMR Structure of the “Ball-and-chain” Domain of KCNMB2, the β2-Subunit of Large Conductance Ca2+- and Voltage-activated Potassium Channels

Content Provider	Journal of Biological Chemistry (JBC)
Author	Bentrop, Detlef Beyermann, Michael Wissmann, Ralph Fakler, Bernd
Description	Synthetic peptide covering the N-terminal 45 amino acids of the β2-subunit of the human BK channel (human KCNMB2) (BKβ2N)
Abstract	The auxiliary β-subunit KCNMB2 (β2) endows the non-inactivating large conductance Ca2+- and voltage-dependent potassium (BK) channel with fast inactivation. This process is mediated by the N terminus of KCNMB2 and closely resembles the “ball-and-chain”-type inactivation observed in voltage-gated potassium channels. Here we investigated the solution structure and function of the KCNMB2 N terminus (amino acids 1–45, BKβ2N) using NMR spectroscopy and patch clamp recordings. BKβ2N completely inactivated BK channels when applied to the cytoplasmic side; its interaction with the BK α-subunit is characterized by a particularly slow dissociation rate and an affinity in the upper nanomolar range. The BKβ2N structure comprises two domains connected by a flexible linker: the pore-blocking “ball domain” (formed by residues 1–17) and the “chain domain” (between residues 20–45) linking it to the membrane segment of KCNMB2. The ball domain is made up of a flexible N terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C terminus. These structural properties explain the functional characteristics of BKβ2N-mediated inactivation.
Related Links	http://www.jbc.org/content/276/45/42116.abstract
Ending Page	42121
Starting Page	42116
Page Count	6
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	45
Volume Number	276
DOI	10.1074/jbc.M107118200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2001-11-09
Access Restriction	Open
Subject Keyword	Large conductance Ca2+ and voltage-dependent K+ channel (BK) Superfamily of voltage-dependent K+ channels (Kv) Nuclear Overhauser effect (NOE) Inactivation domain (ID) 4-morpholineethanesulfonic acid (MES) Root mean square (r.m.s.) PROTEIN STRUCTURE AND FOLDING
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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