NDLI: Molecular Identification and Characterization of Two Medium-chain Acyl-CoA Synthetases, MACS1 and the Sa Gene Product

Content Provider	Journal of Biological Chemistry (JBC)
Author	Fujino, Takahiro Takei, Yumiko A. Sone, Hideyuki Ioka, Ryoichi X. Kamataki, Akihisa Magoori, Kenta Takahashi, Sadao Sakai, Juro Yamamoto, Tokuo T.
Abstract	In this study, we identified and characterized two murine cDNAs encoding medium-chain acyl-CoA synthetase (MACS). One, designated MACS1, is a novel protein and the other the product of the Sa gene (Sa protein), which is preferentially expressed in spontaneously hypertensive rats. Based on the murine MACS1 sequence, we also identified the location and organization of the human MACS1 gene, showing that the human MACS1 and Sa genes are located in the opposite transcriptional direction within a 150-kilobase region on chromosome 16p13.1. Murine MACS1 and Sa protein were overexpressed in COS cells, purified to homogeneity, and characterized. Among C4–C16 fatty acids, MACS1 preferentially utilizes octanoate, whereas isobutyrate is the most preferred fatty acid among C2–C6 fatty acids for Sa protein. Like Sa gene transcript, MACS1 mRNA was detected mainly in the liver and kidney. Subcellular fractionation revealed that both MACS1 and Sa protein are localized in the mitochondrial matrix. 14C-Fatty acid incorporation studies indicated that acyl-CoAs produced by MACS1 and Sa protein are utilized mainly for oxidation.
Related Links	http://www.jbc.org/content/276/38/35961.abstract
Ending Page	35966
Starting Page	35961
Page Count	6
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	38
Volume Number	276
DOI	10.1074/jbc.M106651200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2001-09-21
Access Restriction	Open
Subject Keyword	Acyl-CoA synthetase (ACS) Acetyl-CoA synthetase (AceCS) Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Medium-chain acyl-CoA synthetase (MACS) Polyacrylamide gel electrophoresis (PAGE) Very long-chain acyl-CoA synthetase, RACE, rapid amplification of cDNA ends (VACS) Kilobase(s) (kb) LIPIDS AND LIPOPROTEINS
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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