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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Tanha, Jamshid Xu, Ping Chen, Zhigang Ni, Feng Kaplan, Howard Narang, Saran A. MacKenzie, C. Roger |
| Abstract | We have constructed a human VHlibrary based on a camelized VH sequence. The library was constructed with complete randomization of 19 of the 23 CDR3 residues and was panned against two monoclonal antibody targets to generate VH sequences for determination of the antigen contact residue positions. Furthermore, the feasibility and desirability of introducing a disulfide bridge between CDR1 and CDR3 was investigated. Sequences derived from the library showed a bias toward the use of C-terminal CDR3 residues as antigen contact residues. Mass spectrometric analyses indicated that CDR1-CDR3 disulfide formation was universal. However, surface plasmon resonance and NMR data showed that the CDR3 constraint imposed by the disulfide bridge was not always desirable. Very high yields of soluble protein products and lack of protein aggregation, as demonstrated by the quality of the1H-15N HSQC spectra, indicated that the VH sequence for library construction was a good choice. These results should be useful in the design of VHlibraries with optimal features. |
| Related Links | http://www.jbc.org/content/276/27/24774.abstract |
| Ending Page | 24780 |
| Starting Page | 24774 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 27 |
| Volume Number | 276 |
| DOI | 10.1074/jbc.M100770200 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2001-07-06 |
| Access Restriction | Open |
| Subject Keyword | Single domain antibody (dAb) Complementarity determining region (CDR) 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) Dithiothreitol (DTT) Antigen-binding fragment (Fab) Heteronuclear single-quantum coherence spectroscopy (HSQC) Immunoglobulin G (IgG) Nuclear magnetic resonance (NMR) Nuclear Overhauser effect spectroscopy (NOESY) Phosphate-buffered saline (PBS) Single chain variable fragment of an antibody (scFv) Surface plasmon resonance (SPR) Antibody heavy chain variable domain (VH) Variable domain of a heavy chain antibody (VHH) Antibody light chain variable domain (VL) PROTEIN STRUCTURE AND FOLDING |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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