NDLI: Nuclear Import of the Yeast AP-1-like Transcription Factor Yap1p Is Mediated by Transport Receptor Pse1p, and This Import Step Is Not Affected by Oxidative Stress

Content Provider	Journal of Biological Chemistry (JBC)
Author	Isoyama, Takeshi Murayama, Asako Nomoto, Akio Kuge, Shusuke
Abstract	The yeast AP-1-like transcription factor, Yap1p, is essential for the oxidative stress response in budding yeast. Yap1p is located predominantly in the cytoplasm; however, upon imposition of oxidative stress, Yap1p concentrates in the nucleus and activates target genes. Yap1p is constitutively transported in and out of the nucleus. Oxidative stress inhibits the Crm1p/Xpo1p-dependent nuclear export step, resulting in nuclear accumulation of Yap1p. In this study, we examined the mechanism for Yap1p nuclear import, and determined whether the import step is affected by oxidative stress. The nuclear accumulation of Yap1p required the activity of the small GTPase, Ran/Gsp1p. Under conditions in pse1–1 cells carrying a temperature-sensitive mutation of the importin β family member PSE1/KAP121, nuclear translocation of Yap1p was inhibited dramatically. In an in vitro assay, we showed that Yap1p could directly bind to Pse1p and that this interaction was dissociated by Ran-GTP. These results indicate that Pse1p is the nuclear import receptor for Yap1p. In addition to Pse1p, we suggest that Kap123p, which is homologous to Pse1p, has a minor effect on the nuclear import of Yap1p. Furthermore, we identified the nuclear localization signal of Yap1p and demonstrated that the nuclear import of Yap1p was not affected by oxidative stress.
Related Links	http://www.jbc.org/content/276/24/21863.abstract
Ending Page	21869
Starting Page	21863
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	24
Volume Number	276
DOI	10.1074/jbc.M009258200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2001-06-15
Access Restriction	Open
Subject Keyword	Nuclear pore complex (NPC) Nuclear localization signal (NLS) GTP-loaded form of Ran (Ran-GTP) Green fluorescent protein (GFP) GlutathioneS-transferase (GST) Polymerase chain reaction (PCR) Hemagglutinin (HA) Polyacrylamide gel electrophoresis (PAGE) Wild type (WT) MOLECULAR BASIS OF CELL AND DEVELOPMENTAL BIOLOGY
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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