NDLI: Involvement of the Pro-oncoprotein TLS (Translocated in Liposarcoma) in Nuclear Factor-κB p65-mediated Transcription as a Coactivator

Content Provider	Journal of Biological Chemistry (JBC)
Author	Uranishi, Hiroaki Tetsuka, Toshifumi Yamashita, Mayumi Asamitsu, Kaori Shimizu, Manabu Itoh, Makoto Okamoto, Takashi
Abstract	In this study, we have demonstrated that translocated in liposarcoma (TLS), also termed FUS, is an interacting molecule of the p65 (RelA) subunit of the transcription factor nuclear factor κB (NF-κB) using a yeast two-hybrid screen. We confirmed the interaction between TLS and p65 by the pull-down assay in vitro and by a coimmunoprecipitation experiment followed by Western blot of the cultured cell in vivo. TLS was originally identified as part of a fusion protein with CHOP arising from chromosomal translocation in human myxoid liposarcomas. TLS has been shown to be involved in TFIID complex formation and associated with RNA polymerase II. However, the role of TLS in transcriptional regulation has not yet been clearly elucidated. We found that TLS enhanced the NF-κB-mediated transactivation induced by physiological stimuli such as tumor necrosis factor α, interleukin-1β, and overexpression of NF-κB-inducing kinase. TLS augmented NF-κB-dependent promoter activity of the intercellular adhesion molecule-1 gene and interferon-β gene. These results suggest that TLS acts as a coactivator of NF-κB and plays a pivotal role in the NF-κB-mediated transactivation.
Related Links	http://www.jbc.org/content/276/16/13395.abstract
Ending Page	13401
Starting Page	13395
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	16
Volume Number	276
DOI	10.1074/jbc.M011176200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2001-04-20
Access Restriction	Open
Subject Keyword	Nuclear factor κB (NF-κB) Amino-terminal enhancer of split (AES) CAMP-response element-binding protein (CREB) CREB-binding protein (CBP) Cytomegalovirus (CMV) DNA-binding domain (DBD) Transcriptional activation domain (AD) Glutathione S-transferase (GST) Intercellular adhesion molecule-1 (ICAM-1) Interleukin (IL) NF-κB-inducing kinase (NIK) Nuclear localization signal (NLS) Transducin-like enhancer of split (TLE) Translocated in liposarcoma (TLS) Tumor necrosis factor (TNF) Polyacrylamide gel electrophoresis (PAGE) GENES: STRUCTURE AND REGULATION
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Inhibition of Nuclear Factor-κB-mediated Transcription by Association with the Amino-terminal Enhancer of Split, a Groucho-related Protein Lacking WD40 Repeats

Nuclear Integration of Glucocorticoid Receptor and Nuclear Factor-κB Signaling by CREB-binding Protein and Steroid Receptor Coactivator-1

CREB-binding Protein Is a Nuclear Integrator of Nuclear Factor-κB and p53 Signaling

NF-κB and Enhancer-binding CREB Protein Scaffolded by CREB-binding Protein (CBP)/p300 Proteins Regulate CD59 Protein Expression to Protect Cells from Complement Attack

Transducin-like Enhancer of Split Proteins, the Human Homologs of Drosophila Groucho, Interact with Hepatic Nuclear Factor 3β

CBP (CREB Binding Protein) Integrates NF-κB (Nuclear Factor-κB) and Glucocorticoid Receptor Physical Interactions and Antagonism

β-Catenin Directs Nuclear Factor-κB p65 Output via CREB-Binding Protein/p300 in Human Airway Smooth Muscle

The Groucho/Transducin-like enhancer of split protein family in animal development

NF-κB and Enhancer-binding CREB Protein Scaffolded by CREB-binding Protein (CBP)/p300 Proteins Regulate CD59 Protein Expression to Protect Cells from Complement Attack*

Involvement of the Pro-oncoprotein TLS (Translocated in Liposarcoma) in Nuclear Factor-κB p65-mediated Transcription as a Coactivator

Similar Documents

Inhibition of Nuclear Factor-κB-mediated Transcription by Association with the Amino-terminal Enhancer of Split, a Groucho-related Protein Lacking WD40 Repeats

Nuclear Integration of Glucocorticoid Receptor and Nuclear Factor-κB Signaling by CREB-binding Protein and Steroid Receptor Coactivator-1

CREB-binding Protein Is a Nuclear Integrator of Nuclear Factor-κB and p53 Signaling

NF-κB and Enhancer-binding CREB Protein Scaffolded by CREB-binding Protein (CBP)/p300 Proteins Regulate CD59 Protein Expression to Protect Cells from Complement Attack

Transducin-like Enhancer of Split Proteins, the Human Homologs of Drosophila Groucho, Interact with Hepatic Nuclear Factor 3β

CBP (CREB Binding Protein) Integrates NF-κB (Nuclear Factor-κB) and Glucocorticoid Receptor Physical Interactions and Antagonism

β-Catenin Directs Nuclear Factor-κB p65 Output via CREB-Binding Protein/p300 in Human Airway Smooth Muscle

The Groucho/Transducin-like enhancer of split protein family in animal development

NF-κB and Enhancer-binding CREB Protein Scaffolded by CREB-binding Protein (CBP)/p300 Proteins Regulate CD59 Protein Expression to Protect Cells from Complement Attack*

Involvement of the Pro-oncoprotein TLS (Translocated in Liposarcoma) in Nuclear Factor-κB p65-mediated Transcription as a Coactivator