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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Corey, Shawn Clapham, David E. |
| Abstract | G-protein-coupled inwardly rectifying K+ (GIRK; Kir3.x) channels are the primary effectors of numerous G-protein-coupled receptors. GIRK channels decrease cellular excitability by hyperpolarizing the membrane potential in cardiac cells, neurons, and secretory cells. Although direct regulation of GIRKs by the heterotrimeric G-protein subunit Gβγ has been extensively studied, little is known about the number of Gβγ binding sites per channel. Here we demonstrate that purified GIRK (Kir 3.x) tetramers can be chemically cross-linked to exogenously purified Gβγ subunits. The observed laddering pattern of Gβγ attachment to GIRK4 homotetramers was consistent with the binding of one, two, three, or four Gβγ molecules per channel tetramer. The fraction of channels chemically cross-linked to four Gβγ molecules increased with increasing Gβγ concentrations and approached saturation. These results suggest that GIRK tetrameric channels have four Gβγ binding sites. Thus, GIRK (Kir 3.x) channels, like the distantly related cyclic nucleotide-gated channels, are tetramers and exhibit a 1:1 subunit/ligand binding stoichiometry. |
| Related Links | http://www.jbc.org/content/276/14/11409.abstract |
| Ending Page | 11413 |
| Starting Page | 11409 |
| Page Count | 5 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 14 |
| Volume Number | 276 |
| DOI | 10.1074/jbc.M100058200 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2001-04-06 |
| Access Restriction | Open |
| Subject Keyword | Phosphatidylinositol (PI) G-protein-gated inwardly rectifying K+ channel (GIRK) Dithiobis[sulfosuccinimidylpropionate] (DTSSP) Polyacrylamide gel electrophoresis (PAGE) Inwardly rectifying K+-selective channel (Kir) Native atrial G-protein-gated K+ channel (IKACh) Chinese hamster ovary cells (CHO) 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS) MECHANISMS OF SIGNAL TRANSDUCTION |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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