NDLI: Molecular Cloning of a Novel Scavenger Receptor for Oxidized Low Density Lipoprotein, SR-PSOX, on Macrophages

Content Provider	Journal of Biological Chemistry (JBC)
Author	Shimaoka, Takeshi Kume, Noriaki Minami, Manabu Hayashida, Kazutaka Kataoka, Hiroharu Kita, Toru Yonehara, Shin
Abstract	Receptor-mediated endocytosis of oxidized low density lipoprotein (OxLDL) by macrophages has been implicated in foam cell transformation in the process of atherogenesis. Although several scavenger receptor molecules, including class A scavenger receptors and CD36, have been identified as OxLDL receptors on macrophages, additional molecules on macrophages may also be involved in the recognition of OxLDL. From a cDNA library of phorbol 12-myristate 13-acetate-stimulated THP-1 cells, we isolated a cDNA encoding a novel protein designated SR-PSOX (scavenger receptor that bindsphosphatidylserine and oxidized lipoprotein), which acts as a receptor for OxLDL. SR-PSOX was a type I membrane protein consisting of 254 amino acids, expression of which was shown on human and murine macrophages with a molecular mass of 30 kDa. SR-PSOX could specifically bind with high affinity, internalize, and degrade OxLDL. The recognition of OxLDL was blocked by polyinosinic acid and dextran sulfate but not by acetylated low density lipoprotein. Taken together, SR-PSOX is a novel class of molecule belonging to the scavenger receptor family, which may play important roles in pathophysiology including atherogenesis.
Related Links	http://www.jbc.org/content/275/52/40663.abstract
Ending Page	40666
Starting Page	40663
Page Count	4
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	52
Volume Number	275
DOI	10.1074/jbc.C000761200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2000-12-29
Access Restriction	Open
Subject Keyword	Oxidized low density lipoprotein (OxLDL) Class A scavenger receptors (SR-A) Phorbol 12-myristate 13-acetate (PMA) Human monocyte-derived macrophages (HMDM) Phosphatidylserine (PS) Acetyl LDL (AcLDL) 1,1′-dioctadecyl-3,3,3′3′-tetra-metyllindocarbocyanin perchlorate (DiI) Polyinosinic acid (poly I) Polymerase chain reaction (PCR) Low density lipoprotein (LDL) Scavenger receptor (SR) Chinese hamster ovary (CHO) Kilobase (kb) Expressed sequence tag (EST)
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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