NDLI: Characterization of Proteoglycans of Human Placenta and Identification of Unique Chondroitin Sulfate Proteoglycans of the Intervillous Spaces That Mediate the Adherence ofPlasmodium falciparum-infected Erythrocytes to the Placenta

Content Provider	Journal of Biological Chemistry (JBC)
Author	Achur, Rajeshwara N. Valiyaveettil, Manojkumar Alkhalil, Abdulnaser Ockenhouse, Christian F. Gowda, D. Channe
Abstract	In pregnant women infected with Plasmodium falciparum, the infected red blood cells (IRBCs) selectively accumulate in the intervillous spaces of placenta, leading to poor fetal outcome and severe health complications in the mother. Although chondroitin 4-sulfate is known to mediate IRBC adherence to placenta, the natural receptor has not been identified. In the present study, the chondroitin sulfate proteoglycans (CSPGs) of human placenta were purified and structurally characterized, and adherence of IRBCs to these CSPGs investigated. The data indicate that the placenta contains three distinct types of CSPGs: significant quantities of uniquely low sulfated, extracellular CSPGs localized in the intervillous spaces, minor amounts of two cell-associated CSPGs, and major amounts of dermatan sulfate-like CSPGs of the fibrous tissue. Of the various CSPGs isolated from the placenta, the low sulfated CSPGs of the intervillous spaces most efficiently bind IRBCs. Based on IRBC adherence capacities and localization patterns of various CSPGs, we conclude that the CSPGs of the intervillous spaces are the receptors for placental IRBC adherence. The identification and characterization of these CSPGs provide a valuable tool for understanding the precise molecular interactions involved in placental IRBC adherence and for the development of therapeutic strategies for maternal malaria. In the accompanying paper (Alkhalil, A., Achur, R. N., Valiyaveettil, M., Ockenhouse, C. F., and Gowda, D. C. (2000) J. Biol. Chem. 275, 40357–40364), we report the structural requirements for the IRBC adherence.
Related Links	http://www.jbc.org/content/275/51/40344.abstract
Ending Page	40356
Starting Page	40344
Page Count	13
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	51
Volume Number	275
DOI	10.1074/jbc.M006398200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2000-12-22
Access Restriction	Open
Subject Keyword	Infected red blood cell (IRBC) Chondroitin sulfate (CS) Chondroitin sulfate proteoglycan (CSPG) Heparan sulfate proteoglycan (HSPG) Proteoglycan (PG) Chondroitin 4-sulfate (C4S) Chondroitin 6-sulfate (C6S) Glycosaminoglycan (GAG) Dermatan sulfate (DS) Hyaluronic acid (HA) Bovine serum albumin (BSA) Blue dextran (BD) Glucose (Glc) Hexosamine (HexN) N-acetylgalactosamine (GalNAc) Galactosamine (GalN) Glucosamine (GlcN) Glucuronic acid (GlcA) Guanidine hydrochloride (GdnHCl) N-ethylmaleimide (NEM) 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPS) Phosphate-buffered saline (PBS) Phenylmethylsulfonyl fluoride (PMSF) N-α-tosyl-l-lysine chloromethyl ketone (TLCK) N-tosyl-l-phenylalanine chloromethyl ketone (TPCK) Polyacrylamide gel electrophoresis (PAGE) High performance liquid chromatography (HPLC) Heparan sulfate (HS) GLYCOBIOLOGY AND EXTRACELLULAR MATRICES
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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