NDLI: Functional Complementation of BLNK by SLP-76 and LAT Linker Proteins

Content Provider	Journal of Biological Chemistry (JBC)
Author	Wong, Jane Ishiai, Masamichi Kurosaki, Tomohiro Chan, Andrew C.
Abstract	Recent studies have demonstrated a requirement for the SLP-76 (SH2 domain-containingleukocyte protein of 76 kDa) and LAT (linker for activation of Tcells) adaptor/linker proteins in T cell antigen receptor activation and T cell development as well as the BLNK (Bcell linker) linker protein in B cell antigen receptor (BCR) signal transduction and B cell development. Whereas the SLP-76 and LAT adaptor proteins are expressed in T, natural killer, and myeloid cells and platelets, BLNK is preferentially expressed in B cells and monocytes. Although BLNK is structurally homologous to SLP-76, BLNK interacts with a variety of downstream signaling proteins that interact directly with both SLP-76 and LAT. Here, we demonstrate that neither SLP-76 nor LAT alone is sufficient to restore the signaling deficits observed in BLNK-deficient B cells. Conversely, the coexpression of SLP-76 and LAT together restored BCR-inducible calcium responses as well as activation of all three families of mitogen-activated protein kinases. Together, these data suggest functional complementation of SLP-76 and LAT in T cell antigen receptor function with BLNK in BCR function.
Related Links	http://www.jbc.org/content/275/42/33116.abstract
Ending Page	33122
Starting Page	33116
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	42
Volume Number	275
DOI	10.1074/jbc.M004467200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2000-10-20
Access Restriction	Open
Subject Keyword	Protein-tyrosine kinases (PTKs) Free cytoplasmic calcium concentration(s) ([Ca2+]i) Phospholipase C (PLC) Mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) Extracellular signal-regulated kinase (ERK) Mitogen-activated protein kinase (MAPK) T cell antigen receptor (TCR) B cell antigen receptor (BCR) Monoclonal antibody (mAb) Antibody (Ab) Janus kinase (JNK) MECHANISMS OF SIGNAL TRANSDUCTION
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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