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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Pan, Junmin Snell, William J. |
| Abstract | In the green alga Chlamydomonas reinhardtii flagellar adhesion between gametes of opposite mating types leads to rapid cellular changes, events collectively termed gamete activation, that prepare the gametes for cell-cell fusion. As is true for gametes of most organisms, the cellular and molecular mechanisms that underlie gamete activation are poorly understood. Here we report on the regulated movement of a newly identified protein kinase, Chlamydomonas aurora/Ipl1p-like protein kinase (CALK), from the cell body to the flagella during gamete activation. CALK encodes a protein of 769 amino acids and is the newest member of the aurora/Ipl1p protein kinase family. Immunoblotting with an anti-CALK antibody showed that CALK was present as a 78/80-kDa doublet in vegetative cells and unactivated gametes of both mating types and was localized primarily in cell bodies. In cells undergoing fertilization, the 78-kDa CALK was rapidly targeted to the flagella, and within 5 min after mixing gametes of opposite mating types, the level of CALK in the flagella began to approach levels normally found in the cell body. Protein synthesis was not required for targeting, indicating that the translocated CALK and the cellular molecules required for its movement are present in unactivated gametes. CALK was also translocated to the flagella during flagellar adhesion of nonfusing mutant gametes, demonstrating that cell fusion was not required for movement. Finally, the requirement for flagellar adhesion could be bypassed; incubation of cells of a single mating type in dibutyryl cAMP led to CALK translocation to flagella in gametes but not vegetative cells. These experiments document a new event in gamete activation in Chlamydomonas and reveal the existence of a mechanism for regulated translocation of molecules into an intact flagellum. |
| Related Links | http://www.jbc.org/content/275/31/24106.abstract |
| Ending Page | 24114 |
| Starting Page | 24106 |
| Page Count | 9 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 31 |
| Volume Number | 275 |
| DOI | 10.1074/jbc.M002686200 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2000-08-04 |
| Access Restriction | Open |
| Subject Keyword | Chlamydomonas aurora/Ipl1p-like protein kinase (CALK) Polyacrylamide gel electrophoresis (PAGE) TBS with 0.05% Tween-20 (TBST) Aurora/Ipl1p-related protein kinase (AIRK) MOLECULAR BASIS OF CELL AND DEVELOPMENTAL BIOLOGY |
| Alternative Title | Regulated Targeting of a Protein Kinase into an Intact Flagellum |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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